In Vitro Antiproliferative Activity of Partially Purified Withania Somnifera Fruit Extract on Different Cancer Cell Lines. JBUON 2015; 20(2): 627

مقال فى مجلة

2015

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Purpose: Cancer is a major health problem worldwide. There
is a continuous need to search for safer and more effective
alternatives to overcome the side effects and resistance of the
chemotherapeutic agents. Therefore, in this study we investigated the antiproliferative activity and the apoptotic potential
of Withania somnifera (W. somnifera).
Methods: W. somnifera was extracted with methanol and then
solvent partitioned by sequential extractions with hexane, dichloromethane and ethyl acetate. Each extract was assayed
for antiproliferative activity against different cancer cell lines
using MTT assay. The nuclear morphology of HepG2 cells was
investigated by DNA-binding fluorescent dye (Hoechst 33342
stain). The percentage of viability, death and apoptosis were
evaluated by the TaliTM Image-based cytometer using annexin-V/PI (propidium iodide). A chromatographic fingerprint
was constructed using high performance liquid chromatography (HPLC).
Results: The most potent anticancer activity of the crude extract
was against HepG2 cell line (LC50=164.7μg/ml). Dichloromethane
fraction showed remarkable changes in the chromatin structure
i.e., fragmentation, uniform condensation. Of the HepG2 cells
43.6% were apoptotic when treated with dichloromethane fraction for 24 hrs at 95μg/ml concentration. HPLC showed the presence of a major peak at 11.85 min.
Conclusion: W. somnifera may have the potential to serve as
a template for future anticancer drug development. However,
further investigation is required to identify the active compound/s.

• نوع عمل المنشور: بحث
• اسم الناشر: JBUON