Evaluation of Antiobesity Effect of Mangiferin in Adipogenesis-Induced Human Mesenchymal Stem Cells by Assessing Adipogenic Genes

Journal Article

Alshatwi, P. Subash-Babu and Ali A. . 2014

• رابط المنشور على الويب: http://onlinelibrary.wiley.com/doi/10.1111/jfbc.12101/abstract
• المجلة \ الصحيفة: Journal of Food Biochemistry
• رقم العدد: 1
• رقم الإصدار السنوي: 39
• الصفحات: 28–38

مستخلص المنشور

We aimed to examine the anti-adipogenic effects of mangiferin (xanthone glucoside) and its related mechanism. Human mesenchymal stem cells (hMSCs) were cultured in Dulbecco's modified Eagle medium, after 60% confluence adipocyte differentiation was induced. Differentiation-induced hMSCs were cultured in the presence and absence of 10, 20 and 40 μmol of mangiferin from day 0 to day 10. Adipocyte differentiation and lipid accumulation were significantly decreased in 40 μmol mangiferin-treated groups when compared with the reference drugs (quercetin- and orlistat-treated groups). Using quantitative reverse transcription polymerase chain reaction, we studied the mRNA expression levels of resistin, adipocyte fatty acid-binding protein 2 (aP2), lipoprotein lipase (LPL), peroxisome proliferator-activated receptor (PPAR-γ) and tumor necrosis factor-α, in hMSCs undergoing adipocyte differentiation; treatment with mangiferin attenuated the expression of those adipogenic genes and decreased adipocyte differentiation. Mangiferin significantly inhibited hMSCs to preadipocyte differentiation within the first 2 days of treatment, indicating that the anti-adipogenic effects of mangiferin are achieved through the inhibition of differentiation and maturation.