Basic Microbiology

Department				:	Clinical Laboratory Sciences
Course Number				:	CLS 311
Course Title				:	Basic Microbiology
Credit Hours				:	3 + 1 = 4
Course Description:
This course provides the students with basic theoretical and practical aspects of various groups of microorganisms to include bacteriology, virology, mycology, and parasitology as well as basic concepts of immunology and epidemiology. It also introduces the basic concepts of disinfectants, antiseptics, preservatives, Ames test, methods of sterilization, aseptic techniques and general microbial control.
CLS 311:   Lectures Outline
Weeks		Subjects
1		General introduction to Microbiology
		Historical background and Classification of
		Microorganisms
2		Introduction to Viruses:
		Classification, morphology and structure, Replication and
		Pathogenicity
3		Introduction to Fungi:
		Classification, morphology and structure, Replication and
		Pathogenicity
4		Introduction to Parasites:
		Classification, General Characteristics of parasites and
		Medically important Parasites
5		Introduction to Bacteria:
		Classification, Morphology and Structures
6		Bacterial Structures
7		Microbial Growth requirements:
		Nutritional requirements, Physical requirements,
		Types of bacteria according to energy production
8		Bacterial Growth:
		Growth curve, Constant and synchronous growth
9		Bacterial Metabolism: Catabolic Pathways and regulation
10		Bacterial Genetics:
		Mutation and mutagenic agents ,&Ames test
		Gene transfer ,PCR ,& genetic engineering
11		Bacterial Genetics continued ….
12		Microbial Control:
		Principles, Disinfectants, antiseptics, and preservatives
		Physical and chemical methods of sterilization Aseptic
		techniques
13		Microbial control continued…
14		Pathogenicity of Infectious Diseases
15		Normal Microbial flora
CLS 311:   Laboratory Schedule
Weeks			Subjects
1			Introduction to Microbiology laboratory techniques and
			safety rules
2			Introduction to Microscopy
			Types of Microscopes.
3			Examination of Stained Smear and Wet
			Preparation.
4			Microscopic examination of Eucaryotic microoganisms .
5			Staining of Bacterial Cells ( simple staining )
6			Staining of Bacterial Cells ( differential staining )
7			Preparation and Types of Culture Media
8			Bacterial Culture Techniques
			Colonial Morphology
9			Bacterial Count ( Total & Viable )
10			Factors Affecting Microbial Growth
11			Physical and chemical methods used in microbial
			control
12			Revision
13			FINAL PRACTICAL EXAMINATION
Assessments:
Mid Term Examination: Written						20
Mid Term Examination: Practical						15
Attendance and Reports:						5
Final Practical Examination:						20
Final Theoretical Examination:						40
References:
	1. Cossart, P., P. Boquet, S. Normark, and R. Rappuolo. Cellular Microbiology. ASM Press, Washington, DC, 2000.
	2. Kaufmann, S. H. E., A. Sher and R. Ahmed. Immunology of Infectious Diseases. ASM Press, Washington, DC, 2001.
	3. Madigan, M. T., Martinko, J. M., and J. Parker. Brock Biology of Microorganisms. Prentice Hall, Upper Saddle River, NJ, 1997
	4. Mims, C., A. Nash, and J. Stephen.Mims' Pathogenesis of Infectious Disease. Academic Press, San Diego, CA. 2001.
	5.Prescott,L. M., J. P. Harley, and D. A. Klein. Microbiology (3rd edition) Wm. C. Brown Publishers, Dubuque, IA, 1996.
	6. Salyers, A. A. and D. D. Whitt. Bacterial Pathogenesis. A Molecular Approach. ASM Press, Washington, DC, 1994.
	7. Tortora, G. J., B. R. Funke, and C. L. Case. Microbiology, An Introduction. (7th edition) Benjamin Cummings, San Francisco, 2002.