PHC 427: PHARMACEUTICAL INSTRUMENTAL ANALYSIS

Course Description

The course concerns with the study of the principles and theoretical bases of electrochemical analysis, spectrophotometric analysis as well as separation methods with regard to instrumentation, and application are also included.

PHC 427 : Practical
Lab. No.
I. Ultraviolet and visible spectrophotometry
1 -Beer's Law - recording - stray light effect - calculation
 of constants-deviations - absorabance ratio - log A vs.
 plots - optimum conditions - applications.
2 -Typical benzenoid structures - isosbestic point - solvent
 effect - pH effect - dissociation constant - chromophores
 & auxochromes.
3 -Acid-dye technique - compensation method - correctio
4  II. Optical rotatory dispersion
5 Practical examination.
6  III. High pressure liquid chromatography
i) Factors affecting the separation and analysis 6
 usng HPLC.
ii) Calculation of capacity factor, No. of theoretical
plates and resolution using clobazam stanard and tablets.
Pharmaceutical Applications:
7 Sulphatriad.
Isoniazid tabs or paracetamol paed. drops.
Continuation:
Septrin tabs (Sulphamethoxazole + trimethoprim).
8 Chloramphenicol capsules.
9 Practical exam.
 VI. Electrochemical analysisLab. No.
10 Non-aqueous potentiometric titrations:
-Use of semi-automatic potentiograph for the assay of basic
 medicinal agents in various dosage forms e.g. tablets,
 ointment suppori-tories, oily liquid preparation etc. using
 acetous 0.1 N - perchloric standard solution.
11 Polarography
-Recording of a polarogram of a typical electro-active
 substance e.g. Cd++. Determination of E1/2, residual
 current, diffusion and limiting currents.
-Demonstration of effect of oxygen during polarographing.
-Demonstration of effect of maximum suppressor and
 supporting electrolyte.
Evaluation methods
-Calibration curve method ) Solution of Cd++
 ) and Zn++ are used
-Standard addition method) for illustration.
-Internal standard method)
Amperometric titration
 12 -Pb++ against SO4
-Pb++ against Cr2O7
Bi-amperometric titration (dead-stop)
13 -Various medicinal agents (e.g. sulphonamides)
 are titrated against standard NaNO2 in dil. HCl medium.
-Determination of moisture content in various
 pharmaceutical formulation by Karl Fisher method.
 14 Practical Examination

 

 : Text books

 1.Analytical Chemistry. Gary D. Christian. John Wiley & Sons, New York

           2.  Pharmaceutical analysis: a textbook for pharmacy students and pharmaceutical chemists. D. G. Watson. Churchill Livingstone. Edinburgh

 :Questions

:Mark either true or false for each of the following statements

Isocratic elution the composition is constant ( T )
 HPLC means High performance liquid chromatography ( T ) 
 In Gas chromatography the mobile phase is gas ( T )
Packed columns contain a finely divided, inert, solid support material coated with liquid stationary phase( T )