Obturation Techniques Allow Microbial Leakage Unless Protected
Journal Article
Balto, Hanan . 2016
المجلة \ الصحيفة:
Journal of Prosthodontics
رقم الإصدار السنوي:
25
الصفحات:
224-228
مستخلص المنشور:
Purpose: To evaluate the quality of the apical 5-mm seal produced by different filling techniques using a bacterial leakage model.
Materials and Methods: Sixty-five extracted single-rooted human teeth were deco- ronated, prepared, and instrumented. Roots were randomly divided into three ex- perimental groups (15 roots each) and control groups (10 roots each). The apical 5 mm was filled with cold lateral condensation (CLC) technique, continuous wave of condensation (CWC), or injectable thermoplasticized gutta-percha (Obtura II) using AH26 Plus as a sealer. Positive controls were filled with gutta-percha without sealer, whereas negative controls were filled with a CLC technique and covered completely with two layers of nail varnish, including the orifice. A dual-chamber leakage model using Enterococcus faecalis as a microbial marker was used for leakage evaluation. Bacterial penetration was monitored over a 60-day period, and leakage was recorded when turbidity was observed in the lower chamber.
Results: All positive controls exhibited turbidity in the lower chamber within 24 hours. All negative controls demonstrated no bacterial leakage for the entire 60-day observation period. The estimated mean day for leakage was 32 for CLC, 35 for CWC, and 30 days for Obtura II. Wilcoxon test showed no statistically significant difference (p = 0.98) in the survival time between the experimental groups.
Conclusion: The three filling techniques produced similar resistance to bacterial leakage when used to fill the apical 5-mm segment of the canal while leaving the rest of the canal unfilled.