Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response
Journal Article
Alhazza, Hossam Ebaid*, Bahaa Abdel-salam, Iftekhar Hassan, Jameel Al-Tamimi, Ali Metwalli and Ibrahim . 2015
نوع عمل المنشور:
Post PhD
رابط المنشور على الويب:
المجلة \ الصحيفة:
Lipids in Health and Disease (BioMed Central)
رقم العدد:
DOI 10.1186/s12944-015-0136-9
رقم الإصدار السنوي:
14:132
الصفحات:
1-10
مستخلص المنشور:
Abstract Background: Diabetes mellitus alters oxidative stability and immune response. Here, we investigated the impact of a peptide extracted from camel milk (CMP) on the oxidative status, transcription factor kappa-B (NF-kB) and inflammatory cytokine in diabetic wounds. Methods: Rats were assigned into three groups: control, diabetic induced (DM) and diabetic induced with multiple doses of CMP for a week (DM-CMP). Results: DM showed a sharp decline in the activity of major antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) compared to the control. The DM-CMP group, however, showed a noticeable replenishment in the activity of these enzymes compared to the DM group. The CMP-treated group also showed a normal level of lipid peroxidation marker (MDA) compared to the DM rats. Furthermore, ELISA analysis of serum TNF-α protein showed an elevated level in diabetic rats in comparison to control serum. However, RT-PCR analysis of locally wounded skin tissues revealed that diabetes down-regulates the RNA expression of both TNF-α and MIF genes in comparison to the control samples but that CMP was found to restore RNA expression significantly. Although it was elevated in CMP-treated rats after one day of wound incision, the NF-kB protein level was significantly decreased seven days after the incision in comparison to the animals in the diabetic group. Conclusion: CMP, therefore, can be seen an effective antioxidant and immune stimulant that induces oxidative stability and speeds up wound healing in diabetic model animals, making it a potential adjuvant in improving wound healing in those with diabetic conditions.