Hepatorenal protective effect of Antistax® against chemically‑induced toxicity
Journal Article
Rafatullah, Atallah F. Ahmed, Hanan M. Al‑Yousef, Jawaher H. Al‑Qahtani, Mansour S. Al‑Said, AbdelKader E. Ashour, Mohammed Al‑Sohaibani, Syed . 2015
نوع عمل المنشور:
بحث ماجستير
المجلة \ الصحيفة:
Pharmacognosy Magazine
رقم العدد:
42
رقم الإصدار السنوي:
11
الصفحات:
S173-S181
مستخلص المنشور:
Background: Antioxidant natural products and chemoprevention are considered nowadays as an
effective approach against health various disorders and diseases induced by oxidative stress or free
radicals. Objective: The aim of this study was to assess the hepato‑ and nephroprotective activity of
a standardized red vine leaf aqueous extract AS195 (Antistax®). Methods: The protective activity of
AS195 (100 mg/kg) was investigated on carbon tetrachloride (CCl4)‑intoxicated rats in comparison
with silymarin. The flavonoid/proanthocyanidin nature of AS195 was identified by phytochemical
and nuclear magnetic resonance (NMR) analyses, while its total phenol/proanthocyanidin/flavonoid
content and antioxidant activity were determined by Folin‑Ciocalteau, vanillin‑sulfuric acid, AlCl3,
and 2,2‑diphenyl‑2‑picrylhydrazyl radical scavenging assays, respectively. Results: Relative to
the control CCl4–intoxicated group, pretreatment with AS195 could significantly suppressed the
elevated serum levels of alanine aminotransferase, alkaline phosphatase, g‑glutamyl transferase,
total cholesterol, low‑density lipoprotein cholesterol, very low‑density lipoprotein cholesterol,
triglycerides, bilirubin, creatinine, uric acid, and calcium, whereas it significantly increased
the diminished serum levels of high‑density lipoprotein cholesterol, albumin and total protein.
Moreover, AS195 significantly decreased malondialdehyde formation in the tissues of liver
and kidney, whereas it significantly elevated and nonprotein sulfhydryl groups, compared with
the intoxicated control. The improvement in biochemical parameters by AS195 was obviously
observed and further confirmed by restoration of normal histological features in the two organs.
Conclusions: The results of the present study revealed the capacity of AS195 to enhance the
recovery from xenobiotic‑induced hepatorenal toxicity initiated by free radicals.