Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress.
Journal Article
, Ritesh Kumar, Amit Kumar Singh, Dhruv Lavania, Manzer H. Siddiqui, Mohamed H Al-Whaibi, Anil Grover . 2015
رابط المنشور على الويب:
رقم العدد:
2
رقم الإصدار السنوي:
23
الصفحات:
243–247
مستخلص المنشور:
Heat stress adversely affects the growth and yield of faba bean crop. Accumulation of ClpB/Hsp100 class of proteins is a critical parameter in induction of acquired heat stress tolerance in plants. Heat-induced expression of ClpB/Hsp100 genes has been noted in diverse plant species. Using primers complementary to soybean ClpB/Hsp100 gene, we analyzed the transcript expression profile of faba bean ClpB/Hsp100 gene in leaves of seedlings and flowering plants and in pollen grains. ClpB/Hsp100 protein accumulation profile was analyzed in leaves of faba bean seedlings using Arabidopsis thalianacytoplasmic Hsp101 antibodies. The transcript and protein levels of faba bean ClpB/Hsp100 were significantly induced in response to heat stress.