Effects of 5-Aza-2’ Deoxycytidine on Proliferation and Differentiation of Embryonic Chick Caudal Region Chondrocytes in Culture

Journal Article

Haq, Samina Hyder . 2016

• نوع عمل المنشور: research paper

مستخلص المنشور

Abstract: DNA methylation is one of the epigenetic mechanisms which have been implicated in
cellular differentiation, ageing and disease development. The effect of hypomethylating drug
5-aza-2´deoxycytidine (5-aza dC) on the biosynthetic profile of caudal region chondrocytes from
chick sternum was studied in detail. The chondrocytes in culture were treated with varying doses
of 5-aza dC for 48h and maintained subsequently without the treatment and harvested at
selected time points for analysis of growth and differentiation status. 15μg/ml of 5-aza dC
showed optimum Concentration at which there was a significant increase in DNA synthesis and
RNA synthesis as per cell basis. There was also a significant increase in total protein synthesis and
collagen synthesis as per cell basis at this concentration. This optimal concentration also showed to
up regulate the gene expression of Type X collagen and alkaline phosphatase, which are the marker
of hypertrophic chondrocyte expression. These results further support the notion that methylation
is the major epigenetic factor controlling the differentiation and maturation of chondrocytes.