Enhanced production of Rifamycins B and SV by medium supplementation with Vermiculite

Journal Article

Enshasy, Elsayed A. Elsayed, Hoda Omar and Hesham A. El . 2015

• المجلة \ الصحيفة: J. Chem. Pharm. Res
• رقم العدد: 4
• رقم الإصدار السنوي: 7
• الصفحات: 1089-1094

مستخلص المنشور

Rifamycins are  broad spectrum and important antibiotics used in the treatment of  pathogenic diseases. They are generally  produced  by  the  fermentative  cultivation  of  Amycolatopsis  mediterranei,  using  submerged  cultivation 
system. The medium composition greatly influences the antibiotic production. Trace elements are one ofthe major factors  need  to  be  optimized  to  enhance  the  production  of  rifamycins.  In  the  present  work,  we  investigated  the possible  improvement  of  rifamycins  B  and  SV  production  through  the  addition  of  vermiculite  (dehydrated magnesium-aluminum-ion silicate) as a cheap and affordable source of trace elements. The obtained results showed that the addition of 20 g/L vermiculite gave the highest volumetric production of rifamycins B and SV  (767.5 and 
1265  mg/L for  Rif  B  and SV,  respectively).  Moreover, addition of  vermiculite  at the beginning  of the  cultivation resulted  in  the  highest  volumetric  productivities  in  comparison  to  control  cultivation  (770  and  1305  mg/mL  of rifamycin  B  and SV, respectively). Comparative growth curve kinetics between vermiculite-supplemented  medium and  vermiculite  free  medium  demonstrated  that  vermiculite  significantly  enhanced  cell  growth,  cell  growth  rate, glucose  consumption  rate,  as  well  as  the  production rates  of  both  rifamycins  B  and  SV  compared  to  cultivation without vermiculite. In addition, vermiculite supplementation increased both maximal volumetric productivity and production rates increased by about 32 and 52% for rifamycin B and SV, respectively, as compared to vermiculitefree cultivation.