BCH 320

 (3+0+0)3  Enzymology -320BCH

 

General aspects: nature of enzymes, localization, units of enzyme activity, specificity and specific Nonenzyme catalysts: ribuzymes and abzymes. Enzymes kinetics. Michaelis-Menten equation. Enzyme inhibition. Irreversible inhibition and suicide inhibition. Bisubstrate reaction. Cooperativity and allosteric enzyme. The transition state theory and its analogs. Types of enzymatic catalysis. Definition, classification of coenzymes. Multimolecular form of enzymes. Isolation, purification, characterization of enzymes and criteria of purity of enzymes. Applications of free and immobilized enzymes in the food, and pharmaceutical industries. Enzyme applications in cheese manufacture. Utilization of enzymes baking. Analytical and therapeutic applications of enzymes.