Camel milk peptide improves wound healing in diabetic rats by orchestrating the redox status and immune response

Journal Article

ebaid, Hossam . 2015

• المجلة \ الصحيفة: Lipids in health and disease

مستخلص المنشور

Background: Diabetes mellitus alters oxidative stability and immune response. Here, we investigated the impact of
a peptide extracted from camel milk (CMP) on the oxidative status, transcription factor kappa-B (NF-kB) and
inflammatory cytokine in diabetic wounds.
Methods: Rats were assigned into three groups: control, diabetic induced (DM) and diabetic induced with multiple
doses of CMP for a week (DM-CMP).
Results: DM showed a sharp decline in the activity of major antioxidant enzymes such as superoxide dismutase
(SOD), catalase (CAT) and glutathione (GSH) compared to the control. The DM-CMP group, however, showed a
noticeable replenishment in the activity of these enzymes compared to the DM group. The CMP-treated group also
showed a normal level of lipid peroxidation marker (MDA) compared to the DM rats. Furthermore, ELISA analysis of
serum TNF-α protein showed an elevated level in diabetic rats in comparison to control serum. However, RT-PCR
analysis of locally wounded skin tissues revealed that diabetes down-regulates the RNA expression of both TNF-α
and MIF genes in comparison to the control samples but that CMP was found to restore RNA expression
significantly. Although it was elevated in CMP-treated rats after one day of wound incision, the NF-kB protein level
was significantly decreased seven days after the incision in comparison to the animals in the diabetic group.
Conclusion: CMP, therefore, can be seen an effective antioxidant and immune stimulant that induces oxidative
stability and speeds up wound healing in diabetic model animals, making it a potential adjuvant in improving
wound healing in those with diabetic conditions.