محاضر | محاضرة٫ قسم المختبرات الإكلينيكية٫ كلية العلوم الطبية التطبيقية

العنوان: مبنى ١١، الدور الثالث، مكتب ١١٣

المواد الدراسية

CLS 414
CLS 312
المزيد

جواهر بنت محمد الصغير

التعريف الشخصي: 
محاضرة، قسم المختبرات الإكلينيكية، كلية العلوم الطبية التطبيقية- جامعة الملك سعود

CURRICULUM VITAE

 
EXPERIENCE
2014 - Current University of Cambridge
Ph.D student
  • Extensive characterization of chronic antibody mediated rejection of heart transplant in mouse model.
  • Investigated the effect of antibody affinity maturation on the pathogenesis of cardiac rejection in vivo.
  • Animal techniques.
  • Immunology techniques (ELISA, ELISPOT, western blot).
  • Cell culture, cell migration techniques, and tissue processing (cryostat and IHC).
  • Extensive experience in designing multi-color flow cytometry panels, confocal microscopy, immunoflouresence microscopy, and laser capture microscopy.
  • Molecular biology techniques (PCR, RT-PCR, and sequencing)
2013            King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
  • Trained (6 months) at the stem cells and tissue re-engineering program.
  • Responsible of collecting and processing fresh breast cancer tumor samples from female in-patients.
  • Sort tumor samples for stem cells by FACS.
  • Perform T cell proliferation assays.
  • T cells proliferation measurement by flow cytometry.
  • Perform immunohistochemistry and immunofluorescence staining.
 
2012 – Current               King Saud University, Riyadh, Saudi Arabia.
  • Lecturer at KSU in the field of immunology.
  • Demonstrator of medical microbiology and immunology laboratories.
  • Former head of the KSU internship committee.
  • Current vice-head of the KSU internship committee.
  • Former member of the purchases committee at the microbiology and immunology department.
  • Member of the higher education committee at clinical laboratories department.
 
2009 – 2012                  National Research Council of Canada, Ottawa, ON.
M.Sc Student
  • Isolated anti-CD49d single-domain antibodies from a synthetic VL phage display library by whole cell panning using Jurkat cells.
  • Analyzed the biophysical properties of isolated VLs for isoelectiric point, apparent molecular weight, formula molecular weight, aggregation status, concentration and total protein yield.
  • Expressed and gel-purified novel homodimeric antibody fragments (hFc-VLs).  Each monomer comprised of CD49d-binding VL, hinge, CH2, and CH3 domains using mammalian cell expression system.
  • Gel purified hFc-VLs successfully recognized the native structure of CD49d expressed on transfected K562 cells as confirmed by flow cytometry.
 
2006 – 2007                  King Khaled University Hospital, Riyadh, KSA.
  • Full-time internship in the diagnostic clinical laboratories of King Khaled University Hospital. Completion with Excellence.
  • Trained on different medical laboratory technologies (rotating for one year), including histophathology, medical microbiology, heamatology, and clinical biochemistry.
  • Trained on collecting, processing, and handling venous and capillary blood samples using aseptic techniques.
  • Trained on performing detailed assignments with minimal supervision and work under pressure.
 
 
EDUCATION
 2014 – Current      University of Cambridge, Cambridge, UK
  • Ph.D candidate in Surgery
  • Thesis title “MHC Allo-antibodies and Chronic Heart Rejection”.
  • Finished the following courses:
  • Health and safety training, University of Cambridge.
  • Critical thinking course (2 days, and interaction session), University of Cambridge.
  • Pubmed and endnote course, Addenbrooke’s Hospital, Cambridge.
  • Effective supervision, University of Cambridge.
  • Presentation skills to master, Addenbrooke’s Hospital, Cambridge.
  • Home office animal handelling, CBS, Cambridge.

2009 – 2012         University of Ottawa, Ottawa, ON.

  • M.Sc. in Microbiology and Immunology.
  • Finished the following courses: Host/Pathogen Interactions and Molecular Immunology (A), Immunochemistry (A), and Molecular biology (B+).
  • Independent research for 2 years followed by an oral defence in the area of autoimmunity and antibody engineering.
  • Thesis title: “CD49d-Specific Single Domain Antibodies for the Treatment of Multiple Sclerosis”.
  • Supervisor: Dr. Jamshid Tanha.
 
2002 – 2007                  King Saud University, Riyadh, KSA.
  • Full-time student at the Faculty of Applied Medical Sciences in the field of: Clinical Laboratory Sciences.
  • Degree completed with the second class honour.
 
HONOURS & AWARDS
2016    Second place winner. PhD thesis presentation, 9th SSCUK, 2016 Birmingham, UK
2015    Second place winner. 3-minutes PhD thesis competition, Leeds, UK
2011    Third place winner. University of Ottawa Seminar Day.
2010    Third place winner. University of Ottawa Poster Day.
2009    Scholarship from King Saud University due to achievement of general grade Excellence in B.Sc. Degree.
2007    Achieved the Appreciation Certificate from King Khalid University Hospital for promptness at work and punctuality.
2007    Achieved the Best Student Certificate from King Saud University (for the academic year 2006/2007) for excellent attendance, outstanding academic record, and being an active member of the University’s academic and social community.
2007    Department’s honor list, King Saud University.
 
PUBPLICATIONS
Hussack, G., Keklikian, A., Alsughayyir, J., Hanifi-Moghaddam, P., Arbabi-Ghahroudi, M., van Faassen, H. et al. (2012). A V(L) single-domain antibody library shows a high-propensity to yield non-aggregating binders. Protein Engineering, Design & Selection: PEDS, 25(6), 313-318.

Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant. Cell report, 15 (6), 1214-1227.

 

 
 
 

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