Characterization and gene mapping of a brittle culm mutant of diploid wheat (Triticum monococcum L.) with irregular xylem vessels development

Journal Article

Dhaliwal, Mohammad Javed Ansari • Ahmad Al-Ghamdi • Salma Usmani • Rahul Kumar • Adgaba Nuru • Kuldeep Singh • Harcharan Singh . 2013

• المجلة \ الصحيفة: Acta Physiologiae Plantarum
• رقم العدد: 8
• رقم الإصدار السنوي: 35
• الصفحات: 2407–2419

مستخلص المنشور

Mechanical strength of plants is an important agronomic
trait and directly related to the crop lodging thus yield
losses and grain quality (Ching et al. 2006; Wang et al.
2012). The intrinsic mechanical strength of plants is mainly
controlled by cell wall and any changes in cell wall
structure or composition affect the mechanical strength of
plants (Cosgrove 2000). Sclerenchymatous tissues of plants
having both primary cell wall and secondary thick cell wall
provide the maximum mechanical strength to plants (Carpita
and McCann 2000). Cellulose constitutes 20–30 % of
dry weight of primary cell walls and 40–90 % of the secondary
cell walls (Taylor et al. 1999).
Mutations in the genes of cellulose biosynthesis or
related pathway result in cellulose-deficient mutants or
mutants defective in the stem strength. These mutants are
ideal material to understand the mechanisms that involved
in the mechanical strength of the plant and the biosynthesis
of plant cell wall materials. Several mutants defective in
stem strength were isolated and characterized. Several
brittle culm mutants have been identified in higher plants
and considered excellent material to study the process of
secondary cell wall formation (Aohara et al. 2009). In our
previous study, three EMS-induced brittle culm mutants,
brc1, brc2 and brc3 have been reported in T. monococcum.
These diploid wheat brittle culm mutants exhibit 47–57 %
reduced a-cellulose in the secondary cell walls than that of
WT (Ansari et al. 2012). In Arabidopsis, several mutants
with reduced mechanical strength have been reported
(Turner and Somervilles 1997; Zhong et al. 2005; Wu et al.