السيرة الذاتية
Qualifications
Molecular Genetics and Plant Biotechnology
Education
2006
Ph.D. in Molecular Genetic
Alexandria University: Alexandria, Egypt.
Thesis Title: Genetic studies using biotechnology for the improvement of Potato.
1998
M.Sc. in Plant Biotechnology
Alexandria University: Faculty of Agriculture, Alexandria, Egypt
Thesis Title: Biotechnological studies on Rape (Brassica spp.).
1990
B.Sc. in Genetics
Faculty of Agriculture, Alexandria University,
Alexandria, Egypt.
Professional Experience
Current position
Assistant Prof. of Plant Molecular Biology, Plant Production Department, and Supervisor of the Biotechnology Lab, Faculty of Agriculture, King Saud University.
Previous Projects Member
A member in the following projects:
1. Gene Stacking of the High Molecular Weight Glutenin Genes in Bread Wheat using Molecular Markers (2007 – 2009)
2. The genotypic distribution of ALS-inhibiting herbicides resistant Lolium populations in wheat fields in Saudi Arabia (2008-2011).
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3. Researcher in center of Biotechnology Lab, King Saud University, and a Partner of the team work of the project entitled (Evaluation of genetically modified foods) (2006 – 2007).
4. Member of the research team, Academy Project "Optimization of gene expression in Potato tubers" PI; Prof. Dr. Effat Badr, Department of Genetics, Faculty of Agriculture, Alexandria Univ., Egypt, (2002-2006).
5. Assistant Researcher in a Research Project aiming for the production of wheat lines resistance to the rust diseases employing biotechnological techniques, Faculty of Agriculture, Alexandria University, Alexandria, Egypt (1997 – 1998).
Current Project Member
6. Co-Principal investigator of the Project 11-BIO1828-02 “Deciphering the regulatory mechanisms of abiotic stress tolerance conferred in transgenic plants expressing the AlSAP gene from the halophyte grass A. littoralis” The National Plan for Sciences & Technology.
Training Experience
2011
Targeting Induced Local Lesions IN Genome (TILLING). Organized by Research Team of KSU-INRA Project, College of Food and Agriculture Science, Plant Biotechnology Lab (21th-29th of May).
2008
Training on some Molecular Genetics Techniques such as Gene Isolation and Expression, Faculty of Veterinary Medicine, Ghent University, Belgium (December, 5 – 20).
2005
Participate as trainer of 'Basic Techniques in Molecular Biology' course. Genetics Dep. Fac. Agric., Alex. Univ., Egypt (June, 18-22).
2004
Participate as trainer "Using ELISA, protein analysis and PCR-based Techniques. In Diagnosis of Plant Diseases" course. Biotechnology Lab., Plant Pathology Research Institute, Agriculture Research Center, Alex., Egypt (May, 7-18).
2004
Participate as trainer of "Molecular biology techniques and its using in molecular plant pathology" course. Biotechnology Lab., Plant Pathology Research Institute, Agriculture Research Center, Alexandria, Egypt (Sep., 4-15).
2000-2001
DSE Fellowship, REGIA Project to investigate the structure and function of Arabidopsis; Isolation and cloning of different gene-fragments assigned
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as transcriptional factors. PI: Dr. Helmut Baeumlein, Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
2000
Participate as trainer of "Analytical techniques for research, industry and Environmental studies" course. Biotechnological Center, Alex. Univ., Egypt (Sep., 2-7).
1999-2001
Member of the research team, Univ. Linkage project: "Genetic engineering in Potato for enhanced crop value in Egypt". PI: Prof. Effat Badr. Dept. of Genetics, Fac., Agric., Alex. Univ., Egypt.
1999
Participate as trainer of "Tissue culture techniques". PCR basic and different blotting techniques. Faculty of Agric; Alexandria University (May, 18).
1999
Participate as trainer of: Biotechnology and Genetic Engineering in Industrial Fungi and Higher Plant. Biotechnology Center, Alex. Univ., Egypt (July, 7-17).
Awards
DSE Fellowship: (Nov. 1, 2000 - Nov. 2001), "Utilization of Plant Genetic
Resources as a Contribution to Food Security", Germany. Academic Experience
Participated in teaching practical course for undergraduate students in field of Genetics and Biotechnology, Department of Plant Production, College of Food and Agricultural Sciences, King Saud University (2007- until now). Teaching practical course concerned Advanced Genetics, for Post-graduate students in Department of Plant Production, College of Food and Agricultural Sciences, King Saud University (from 2012 until now). Teaching practical course concerned Lab Techniques, for Post-graduate students in Department of Plant Production, College of Food and Agricultural Sciences , King Saud University, (2012-Present).
Technical Experience
Plant Tissue culture and regeneration.
Protoplast fusion.
DNA and RNA isolation from various samples.
Plasmid isolation.
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Gel electrophoresis of DNA and RNA.
Construction of plasmids for in vitro transcription.
SDS-PAGE of protein.
PCR.
Real-Time PCR
Southern hybridization.
DNA and RNA mobility shift assays.
Cloning of DNA in E.coli.
Reverse transcription and cDNA synthesis.
DNA molecular marker techniques (RAPD).
Agrobacterium- mediated gene transfer.
Chromatography analysis.
Computer Skills
Word Processing; Word perfect, MS word.
Data preparation and presentation: Power Point, Adobe Photoshop.
Data documentation: Gel Documentation Imaging System.
Data mining and submission in databases.
Nucleic acids and protein alignment.
Membership
Membership in Arab Society for Plant Protection.
Membership in Saudi Biological Society.
Research Interes
Differential expression of nitrogen use efficiency in Barely
Mapping of the quantitative trait loci (QTL) for cereal cyst nematode resistance genes in spring wheat.
Posters and Oral Presentation 1) A.I. Ghazy, A.M. Zakri, M.T. Sadder, A.A. Alsadon, A.A. Al-Doss. 2014. Differential Gene Expression in Bitter Apple (Citrullus colocynthis L.) under Salinity Stress. Applied Vegetable Genomics, Vienna, Austria (February, 19-20).
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2) Ghazy, A.I., A.A. AL-Doss, S. Alfaifi, H. Migdadi, A. Salem. 2013. The genotypic distribution and molecular bases ALS-inhibiting herbicides resistant Lolium populations in wheat fields in Saudi Arabia. The International Conference on the status of Plant and Animal Genome XXI, San Diego, California- USA (January 12- 16).
3) Barakat, M.N., A.A. Al-Doss, A.I. Ghazy, K.A. Moustafa and A.A. Elshafei. 2013. Production of Doubled Haploid Lines with High-Molecular-Weight Alleles via Microspore Cultures in Wheat. Plant and Animal Genome XXI Conference. Plant and Animal Genome, San Diego, CA, USA.
4) Ghazy, A.I., K.A. Mostafa and A.A. Al-Doss. 2012. Development New Spring Bread Wheat Lines for Improved Bread Making Quality using Marker Assisted Seletion. Plant and Animal Genome XX, San Diego-USA 14-18 January 2012.
5) Ghazy, A.I., I.M. Moussa and A.A. Al-Doss. 2011. Multiplex Polymerase Chain Reaction for Detection and Characterization of Shiga Toxigenic Escherichia coli (STEC). The 26th Meeting of Saudi Biological Society, Climatic Change and Biodiversity, at Taif University, Taif (24 April).
6) Ghazy, A.I., Y.E. Mabrouk, E. Badr and F. Rakha. 2008. Differential Expression of Potato WRKY1 Gene Upon Erwinia carotovora Infection and Bioinformatics Analysis for a Novel Tomato WRKY-like Gene. The 24th Meeting of the Suadi Biotechnology Society title “Biotechnology Reality and Applications” from 7-9 April, in Cooperation with Tiabah University and Saudi Society of biotechnology, Madinah, KSU. (Oral presentation)
7) Ghazy, A.I., E. Badr, Y. Mabrouk and F. Rakha. 2008. Agrobacterium tumefaciens-Mediated Transformation of Potato and Analysis of Genomic Instability by RAPD. The 24th Meeting of the Suadi Biotechnology Society title “Biotechnology Reality and Applications” from 7-9 April, in Cooperation with Tiabah University and Saudi Society of biotechnology, Madinah, KSU. (Oral presentation)
8) Zeid, M., A.I. Ghazy and W. Link. 2006. Questing for Orobanche crenata resistance genes in faba bean. In: Biovision, 26-29 April, 2006, Alexandria, Egypt.
Conferences
2013
The International Conference on the status of Plant and Animal Genome XXI, San Diego, California- USA (January 12- 16).
2012
The International Conference on the status of Plant and Animal Genome
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XX, San Diego, California- USA (January 15-19).
2009
The International Conference on the status of Plant and Animal Genome XIX, San Diego, California- USA (January 15-19).
2009
The 24th Meeting of the Suadi Biotechnology Society title “Biotechnology Reality and Applications” from 7-9 April, in Cooperation with Tiabah University and Saudi Society of biotechnology, Madinah, KSU.
2009
The first International Conference in Biotechnology (Towards Knowledge-Based Economy), from 16-18 February, Center of Excellence in Biotechnology Research, King Saud University, Riyadh, KSU."
2008
The 4th International Conference on Water Resources and Arid Environment, from 18-21 March, Prince Sultan Research Center for Environment, Water and Desert, King Saud University, Riyadh, KSU.
2007
The 3th International Conference on Water Resources and Arid Environment, from 16-18 November, Prince Sultan Research Center for Environment, Water and Desert, King Saud University, Riyadh, KSU.
2007
Workshop on Biotechnology in the Kingdom of Suadi Arabia. September 10, Center of Excellence in Biotechnology Research, King Saud University, Riyadh, King Saud University, KSU.
2004
Biovision-, Alexandria (The New Life Sciences Ethics patens and the Poor) from 3-6 April, Alexandria, Egypt.
2002
Biotechnology and Sustainable Development Voices of the South and the North from 16-20 March. Alexandria, Egypt.
2001
Tagung der Gesellschaft für Pflanzenzüchtung e. V.; 9. Tagung der AG Molekulare Marker in der Gesellschaft für Pflanzenzüchtung gemeinsam mit dem Scientific Coordinating Committee (SCC) von GABI. 25-26 Sept., Halle, Germany.
Sequences Submitted to the gene Bank
Al-Doss,A.A., Al-Faifi,S.A., A.I. Ghazy, A.K. Salem, M.H. Ammar, M.A. Khan and H.M. Migdadi. 2013. Molecular evidence for new ALS-resistance alleles in Saudi Lolium rigidum populations. 15 Sequences submitted on 9 July, 2003 (direct submission). PopSet: 566560676
http://www.ncbi.nlm.nih.gov/popset?DbFrom=nuccore&Cmd=Link&LinkName=nucc...
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Publications
1) Al-Doss, A.A., A.I. Ghazy, A.K. Salem, H.M. Migdadi and S.A. Al-Faifi. 2013. Identification and Distribution of ALS resistant Lolium rigidum populations in Saudi.Arabia. Journal of Food, Agriculture & Environment 11 (3&4):1311-1314.
2) Barakat, M.N., A.A. Al-Doss, A.A. Elshafei., A.I. Ghazy, K.A. Moustafa. 2013. Assessment of genetic diversity among wheat doubled haploids using TRAP markers and morphoagronomict traits. Australian Journal of Crop 7(1):104-111.
3) Ghazy, A.I., A. Zanouny, K.A. Moustafa and A.A. Al-Doss. 2012. Molecular Screening of High Molecular Weight Glutenin Genes in Spring Bread Wheat Genotypes in Saudi Arabia. Journal of Food, Agriculture & Environment 10 (1): 132-136.
4) Zeid, M. and A.I. Ghazy. 2011. Screening for Resistance Gene Analogs in the Faba been Cultivar Giza 402. Egypt. J. Plant Breed 15 (2): 207-214, Special Issue.
5) Moussa, I. M., A.A. Al-Qahtani, M.A. Gassem, A.I. Ghazy and A.M. Shibl. 2011. Pulsed-Field Gel Electrophoresis (PFGE) as an Epidemiological Marker for Typing of Methicillin Resistant Staphylococcus aureus Recovered from KSA. African Journal of Microbiology Research 5(12): 1492-1499.
6) Mabrouk, Y., E. Badr, F. Rakha and A.I. Ghazy. 2008. Differential Expression of Potato WRKY1 Gene upon Erwinia carotovora Infection and Bioinformatics Analysis for a Novel Tomato WRKY-like Gene. Australian Journal of Basic and Applied Sciences 2(1): 30-36.
7) Badr, E., Y., Mabrouk, F. Rakha and A.I. Ghazy. 2008. Agrobacterium tumefaciens-Mediated Transformation of Potato and Analysis of Genomic Instability by RAPD. Research Journal of Agriculture and Biological Sciences 4(1): 16-25.
8) Tahoun, M.K., A.I. Ghazy and A.E. Ali. 1999. Oil and erucic contents of five Brassica species grown for four successive years in Egypt. 10th International Rapeseed Congress, 26-29 Sept. 1999, Canberra, Australia.
9) Ali A.E., A.I. Ghazy and M.K. Tahoun. 1999. The influence of Brassica species and accession on productivity and nutrient quality of forage rape in Egypt. 10th International Rapeseed Congress, 26-29 Sept. 1999, Canberra, Australia.
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Manuscript under publication
10) Barakat, M.N., A.A. Al-Doss, A.I. Ghazy, K.A. Moustafa and A.A. Elshafei. 2015. Production of doubled haploid lines with high- molecular-weight alleles via microspore cultures in wheat (submitted).
11) Ghazy, A.I., K. A. Moustafa and A.A. Al-Doss. 2015. Developing New Bread Wheat Lines for Improved Bread Making Quality using Marker Assisted Selection.
12) Al-Doss, A.A., A.K. Salem, A.I. Ghazy, H.M. Migdadi and S.A. Al-Faifi. 2015. Performance of Hussar® and Atlantis® ALS herbicides to control ryegrass weeds in wheat fields in Saudi Arabia.
13) Al-Doss, A.A., A.I. Ghazy, A.K. Salem, H.M. Migdadi and S.A. Al-Faifi. 2015. Survey of ALS Herbicide Resistant in Ryegrass (Lolium rigidum) Populations in Saudi Arabia (submitted).
14) Al-Doss, A.A., A.I. Ghazy, A.K. Salem and M.H. Ammar. 2015. Identification and distribution of ALS-herbicides resistant alleles of L. rigidum, in Saudi Arabia (submitted).
15) Al-Doss, A.A., A.I. Ghazy, A.K. Salem and M.H. Ammar. 2015. Development of EST-SSR and molecular diversity assessment of ray grass populations grown in Wheat fields of Saudi Arabia.