Microbial leakage of Cavit, IRM and Tem Bond in post prepared root canals using two methods of gutta-percha removal: In vitro study
The aim of this study was to evaluate the integrity of the coronal seal of Temp-Bond and compare it to
Cavit and IRM after post space preparation using S. faecalis as a microbial tracer. In addition, the affect
of two methods of gutta percha removal on the apical seal of root canal fillings was also evaluated. Forty
extracted human single rooted teeth were prepared chemomechanically and obturated with gutta percha and
AH26 sealer cement using the lateral cold condensation technique to a standardized working length of 15
mm. About 10 mm of the coronal gutta-percha was removed with either Peeso-reamer or a hot plugger. The
roots were divided into three experimental groups of 10 roots and a control group. Each experimental group
was subdivided equally into two groups of 15 each according to the method of post space preparation. Cavit,
IRM, and Temp-Bond were used to seal the access opening. Each root was fixed in a cuvette containing
Tryptic Soya Broth which, covered 2 mm of the root apex. Bacterial suspension was introduced through
pipette. Fresh bacterial suspension was added every week, and the system was monitored daily for the growth
of microorganisms for a period of one month. The results showed there was no significant difference in terms
of coronal leakage between the three coronal materials used (P=0.478), but the methods of gutta-percha
removal did have an impact on the apical leakage (P=0.047). The mean value showed the Peeso-reamer
provided less leakage compared to using a hot plugger during the 30-day experimental time period. It was
concluded the temporary type of coronal seal of endodontically treated teeth will not prevent coronal leakage if
left for a long period of time. In addition, permanent cementation of the post with the coronal restoration should
be carried out as soon as possible to prevent recontamination of the root canal.
Keywords: S. faecalis, leakage of Cavit, IRM and Temp-Bond, gutta-percha removal
