تقنية مختبرات عملية حين 461

ارشادات السلامة في المختبرات , حيوانات التجارب و طرق اخذ العينات و اعطاء الجرعات طرق الفصل الجزيىات و تقنياتها , دراسة مكونات الدم و الخلايا و عد انواعها , فحوصات المضادات الحيوية , دراسة التفريد الكهربائي , تحديد البروتين الكلي

 

Q (1) : Match between  (A) & (B) :

 

A	Terms	B	Definitions
1	Confluent		Number of cells per cm3 of substrate.
2	Adherent culture		Occurred during work .
3	Cell line		Transfer the cells from one tube to another , or from flask to another .
4	Cell density		Spleen cells
5	Passage		A culture after the first sub culture .
6	Primary Culture		Liver cells
7	Suspension culture		Where all the cells are in contact all around there periphery with other cells and no available substrate is left uncovered .
8	Cell concentration		It's the first culture direct from the organ.
9	Contamination

 

 

 

 

 

 

 

 

 

 

 

Q (2) :Give a reason :

1-   Using ice while homogenizing liver and spleen.

 

 

 

 

2-   Shaking the flasks after seeding the cells and before putting them in the incubator.

 

 

 

 

3-   The addition of Trypsin while subculturing the adherent cells.

 

 

 

 

 

 

4- Loosing the caps of the tissue culture flasks before putting them in the CO₂ incubator.

 

 

 

 

 

 

Q (3):Write in :

(a)         Source of contamination ?

1-

2-

 

(b)        Type of tissue culture?

1-

2-

3-

 

(c) Blood components?

 

 

 

 

 

 

(d)        Mention the types of media that we used in culture of bacteria.

1-

2-

(e)2.The difference in culture between bacteria and fungi.

 

 

 

 

Q (4):Fill in the blanks within the following table:-

a)   1-Identify specimen (A) and (B).

b)   What are the possible slides that we can made from each one?

Slides  Possible	Name of specimen
		A
		B

 

 

 

 

 

 

 

Good Luck .. ..