السيرة الذاتية
التعريف الشخصي:
Educational Qualification:
Degree : Ph.D (Biosciences) in 2008
University : Jamia Millia Islamia (Central University), New Delhi, India
Thesis : Development and application of a protocol for detection, prevention and elimination of Mycoplasma contamination in cell cultures.
Present Position and : Researcher
Place of work Vitiligo Research Chair, College of Medicine & King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia
Research specialization (Major scientific fields of interest):
I. Keratinocyte (adult epidermal stem cell) Cell Biology:
(a) Isolation and setup of primary human Keratinocyte (adult epidermal stem cell) culture for application in burn’s patients for the wound closure.
(b) In vitro cultured human epidermis sheets were evaluated and confirmed by light & transmission electron microscopy and immunohistochemistry before grafting to the burn’s patients.
(c) Human and mouse dermal fibroblasts culture and its attenuation with Mitomycin C & further use for supporting the growth of human differentiated Keratinocyte.
II. Mycoplasma in Cell Cultures:
(a) Screening of adult epidermal stem cells (keratinocytes) for any micro-organisms including mycoplasma contamination by various detection methods before autologous grafting of epidermal sheets to the burn’s patients for wound closure.
(b) Detection & identification of mycoplasma contamination in routinely used cell cultures.
(c) Study the toxic effects and application of different anti-mycoplasma agents and antibiotics to eradicate mycoplasma contamination from cell cultures.
III. Pigment Cell Research:
(a) Isolation and setup of primary melanocytes culture from foreskin.
(b) Low level laser (red, blue & UV) irradiation to melanocytes for enhancing their growth & melanogenesis.
(c) Treatment of melanocytes with various plant extracts for enhancing their growth & melanogenesis.
IV. Cancer Biology: Low level laser (red, blue & UV) irradiation to SK-MEL-28 melanoma cancer cell line for reducing their growth for treatment of melanoma cancer.
V. Stem Cell Research: Low level laser (red, blue & UV) irradiation to Adipose-derived Stem cells and human embryonic stem cells derived mesenchymal stem cells for enhancing their growth & their use in regenerative medicine.
Technical skills: Animal cell culture techniques, sub-culturing, cryo-preservation, cell lines maintenance, MTT assay, cell migration assay, invasion assay, detection of mycoplasma contamination in cell lines, PCR, gel electrophoresis (horizontal & vertical), gel-doc imaging system, immunoflorescnce assay, immunohistochemistry, sample processing for transmission electron microscopy, animal handling, treatment of various cell lines with low-level lasers and anti-cancer agents, Quantitative Real-time PCR, western blot, quantification of melanin content from melanocytes.
Patent: Yerneni & Kumar (2009): A culture system for the growth of stem cells. Indian Patent File No.2086/DEL/2009. (Filing date 7/10/2009, Publication date 13/8/2010, Issue 33/2010, p 24092. (Annual report, National Institute of Pathology 2009-10 pp. 167; www.instpath.gov.in).
http://ipindiaonline.gov.in/Eregister_Ipairs/eregisternew/eregisterreport.aspx?patent=2FQM2Nq1LKXKoFuqrtjSJw==&loc=+mN2fYxnTC4l0fUd8W4CAA==
http://ipindiaonline.gov.in/patentsearch/GrantedSearch/ReportProjectPopUp.aspx?Appl_No=L4gFXeUTCLT1YOYCBTNQNA==&Pbl_No=L4gFXeUTCLT1YOYCBTNQNA==&Pat_No=2FQM2Nq1LKXKoFuqrtjSJw==
Research Project was sanctioned from Basic Medical Science division (ICMR) as a Sr. Res. Fellowship and completed successfully:
Cell culture contamination with mycoplasma in basic and applied biomedical research Project Number 2004-03570; Subject Cellular and molecular biology; funded by Indian Council of Medical Research (ICMR), Ministry of Health & Family Welfare, Govt. of India. Start Date 2005-09-01
End Date 2008-08-31.
Research Project was sanctioned from National Plan for Science and Technology (NPST), King Saud University as a Co-Investigator:
Effects of low level lasers on the proliferation, differentiation and calcium deposition of human embryonic stem cells derived mesenchymal stem cells. (Project ID: 13-MED1390-02)
Completed research projects as Senior Research Fellow:
- In vitro cultivation of differentiated epidermis from human Keratinocytes suitable for autologous grafting in burns patients. Annual report of National Institute of Pathology (NIP), New Delhi, India 2003-04 pp. 76-79; (http://icmr.nic.in/annual/iop/2003-04/r4.pdf).
- Cell culture contamination with mycoplasma in basic and applied biomedical research. NIP 2004-05 pp. 103-106; 2005-06 pp. 92-94; 2007-09 pp. 137-139; (http://www.icmr.nic.in/annual/2004-05/iop/misc_projects.pdf & http://www.icmr.nic.in/annual/2005-06/iop/misc_projects.pdf).
- Optimal attenuation conditions for 3T3 fibroblasts for use as feeder cells. NIP 2007-09 pp. 127-129; 2009-10 pp. 137-138; www.instpath.gov.in.
Completed research projects as Post-doctor (at King Saud University):
(1) The vascular sclerosing effects of Bleomycin on varicose veins: A prospective clinicopathological study on experimental animals. It was sanctioned from KACST, Saudi Arabia.
(2) The vascular sclerosing effects of Phenol on varicose veins: A prospective study on experimental animals. It was sanctioned from KACST, Saudi Arabia.
(3) A comparative study of the effect of various wavelengths of low-level laser therapy on the growth of human melanocytes in vitro". Sanctioned from NPST; Project ID: 10-MED1222-02, King Saud University, Riyadh.
ملف مرفق:
| المرفق | الحجم |
|---|---|
 curriculum_vitae_of_dr_ashok_kumar.doc | 127 كيلوبايت |