The three- vessel occlusion as a model of vascular dementia – oxidative stress and mitochondrial failure as an indicator of brain hypoperfusion.
Book Chapter
, Aliev G, Horecký J, Vancová O, Ashraf GM, Hassan I, Bragin V, Bragin I, Shevtsova E, Klochkov SG, Kosenko EA, Cacabelos R, Bachurin SO, Kaminsky YG. . 2014
نوع عمل المنشور:
Post PhD
مدينة النشر:
Berlin Heidelberg
رابط المنشور على الويب:
اسم الجهة الناشرة:
Springer
عنوان الكتاب:
Systems Biology of Free Radicals and Antioxidants
الصفحات:
2023 to 2032
مستخلص المنشور:
Brain energy disorders and oxidative stress due to chronic hypoperfusion are considered to be major risk factors in the pathogenesis of dementia . The aim of our study was to evaluate changes of braincreatine kinase (BB-CK) reaction and mitochondrial respiratory chain function in male Wistar rats exposed to chronic cerebral hypoperfusion. Three-vessel occlusion (3-VO) was accomplished without thoracotomy using a minimally invasive surgical approach for the occlusion of the brachiocephalic trunk and the left common carotid artery (CCA ). The forward rate constant of creatine kinase (kfor) was measured in vivo by saturation transfer of 31P magnetic resonancespectroscopy (MRS) at 2 and 10 weeks of permanent 3-VO. The function of the mitochondrial respiratory chain in vitro was assessed polarographically at 10 weeks after 3-VO. As compared to the controls, the significant 42 % reduction of kfor at 2 respiration. 10 weeks indicated disorders in brain energy metabolism , which is in agreement with the 12 % decrease of the oxidative phosphorylation coefficient (ADP :O) and with the 14 % decrease of the oxidative phosphorylation rate (OPR) measured in isolated mitochondria obtained from the hippocampal tissue . Oxidative modification of the creatine kinase system (inactivation of enzymes ) and metabolic disorder s due to chronic 3-VO, thus, may participate in vascular cognitive impairment and neuronal degeneration.
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