Candida albicans is the most numerous commensal and potentially pathological yeast in the human oral cavity. Here we investigate the ability of C. albicans to form a biofilm on human root tip surfaces (RTs) in the presence of periodontal ligament (PDL) cells, and we compare the expression of cytokines in PDL cells induced by biofilm on RTs compared to that induced by planktonic C. albicans. PDL cells were added at the adhesion phase of C. albicans, or after C. albicans cells were allowed to attach to RTs for 90 minutes. The whole mixed culture was allowed to form a biofilm.

OBJECTIVE:

Candida albicans is the most numerous commensal and potentially pathological yeast in the human oral cavity. The purpose herein is to investigate the ability of C. albicans to form a biofilm in the presence of periodontal ligament (PDL) fibroblasts.

MATERIAL AND METHODS:

Osteoradionecrosis of the jaws is one of the most serious oral complications of head and neck cancer radiation therapy. It is a self‑progressive process of bone destruction that is very difficult to treat. In this report, four patients who are susceptible to osteonecrosis of the jaw presented for endodontic management of nonrestorable teeth. The first patient had two molar teeth with necrotic pulp and was treated with nonsurgical root canal therapy (NSRCT) followed by crown amputation and hemisection.