The study was designed to find out the protein complex(s) associated with HDAC3 in liver cancer using a modified form of affinity purification coupled with a mass spectrometry technique in HepG2 cells. The organ-specific requirement for HDAC1 and HDAC3 during liver formation in zebrafish and their altered expression in liver cancer tissues indicates they are indispensible for hepato-organogenesis and hepatocarcinogenesis. However, how they exert their function is unknown.

MATERIAL & METHODS:

قادت التقانة الرقمية إلى تطورات كبيرة في المجالات المختلفة، ويمكن وصف العصر الحالي بالعصر الرقمي.
لقد تمت الإشارة -في الجزء الأول من هذه المقالة-  إلى أن التعليم في هذا العصر سيعتمد على المدرسة الإلكترونية، حيث التقانة الجديدة من الحواسب والأجهزة المرتبطة بها والشبكات وخاصة الإنترنت ستكون أدوات شائعة. وستؤدي هذه التقانة إلى تغير جذري في العملية التعليمية.
 وفي الجزء الثاني تم تسليط الضوء على التوجهات والمبادرات والقرارات العائدة للمدرسة الإلكترونية/مدرسة التعليم المستقبلي في بلدان الاتحاد الأوربي.

Series of Schiff bases of valproic acid hydrazide,

N

-valproylglycine hydrazide and

N

-valproyl-4-ami

-

nobenzoyl hydrazide derivatives were synthesized and characterized by IR, NMR (

1

H- and

13

C-NMR) and

elemental analysis. The prepared compounds were evaluated in transgenic zebrafish embryos (Tg:

flil-1

: en

-

hanced green fluorescent protein (EGFP)) for antiangiogenic activity and in HepG2 liver carcinoma cell line

for anti cancer activity. The Schiff bases of

N

Pulicaria genus belongs to the family Compositae. Some species of this genus have been used in traditional Arab medicine as antispasmodic agent. In this study the Pulicaria glutinosa, which is a wild growing shrub in Saudi Arabia have been explored for developmental toxicity using zebrafish embryos. The fractionated crude extract showed diverse biological activity as methanol fraction exhibited highest antioxidant activity (IC50 12.2?0.27%) followed by chloroform fraction (18.1?0.36%), while hexane fraction did not show any antioxidant activity.

Pulicaria is a genus of flowering plant in the Asteraceae family. It contains over 100 species. In this study the crude extract was prepared from the fresh leaves of P. glutinosa and further fractionated into three solvent to solvent fractions. The zebrafish (Danio rerio) embryos were treated with these extracts to explore the developmental toxicity. The crude extract did not show any significant toxicity; however, hexane fraction induced severe neurotoxicity in zebrafish embryos at 5 μg/mL. The brain formation was severely impeded and sever apoptosis was observed in treated embryos.