Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of
cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of C. angustifolia in
calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl2 ∙ 2H2O were found most suitable for
encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medium
supplemented with thidiazuron (5.0 μM) + indole-3-acetic acid (1.0 μM) produced maximum number

An effective and improved plant regeneration system was successfully developed using shoot tip explants taken from a two years old mature plant of Cassia angustifolia. The effect of different cytokinins, [6-benzyladenine (BA), Kinetin (Kin) and thidiazuron (TDZ)] at different concentrations (0.5-10 μM) were evaluated as augmented with Murashige and Skoog (MS 1962) medium. Among all the cytokinins tested, TDZ (5.0 μM) was optimum in inducing multiple shoots as compared to BA and Kin.