A new 5-hydrazono[1,2,4]triazolo[1,5-a]quinazoline series was synthesized by the reaction of 5-hydrazino[1,2,4]-triazoloquinazoline with different aryl(hetero)ketones or acid anhydrides and their structures were established using NMR and HREI-MS analyses. The potential cytotoxicity of all synthesized compounds against the human breast (MCF-7), hepatocellular (Hep-G2), human cervical (HeLa) and mouse fibroblast (L929) cell lines was evaluated in vitro using MTT assay.

In a continuation of and update to our research on the synthesis and biological evaluation of 1,2,4-triazolo[1,5-a]quinazolines, this study deals with a new series of their 5-hydrazono-[1,2,4]triazolo[1,5-a]quinazolines (1-19) that was synthesized by the reaction of 5-hydrazino-[1,2,4]triazoloquinazolines with a variety of aryl(hetero)aldehydes. Their cytotoxic effects have been evaluated in vitro in relation to human breast MCF-7, hepatocellular Hep-G2, human cervical HeLa and mouse fibroblast L929 cancer cell lines, using MTT assay.

Oxytetracycline (OTC) production by  Streptomyces rimosus  was studied in batch and fed-batch cultures in shake flask and bioreactor levels using semi-defined medium. First, the effect of glucose concentration on OTC production and growth kinetics was studied intensively. The optimal glucose concentration in the medium was 15 g/L. Higher glucose concentrations supported higher biomass production by less volumetric and specific antibiotic production. Based  on  these  data,  cultivations  were  carried  out  at  semi-industrial  scale  15  L  bioreactor  in  batch  culture.

N-Heterocyclic carbene (NHC) metal complexes possess diverse biological activities but have yet to be extensively explored as potential chemotherapeutic agents. We have previously reported the synthesis of a new class of NHC metal complexes N-heterocyclic with acetate [IPr(BIAN)AuOAc]and chloride [IPr(BIAN)AuCl] ligands. In the experiments reported herein, the zebrafishembryos were exposed to serial dilutions of each of these complexes for 10–12 h. One hundred percent mortality was observed at concentrations ≥50 µM.

Fructooligosaccharides have been mainly produced by microbial fructosyltransferases (FTase) enzymes. The present work focuses on the optimization of medium composition and cultivation parameters affecting FTase produced by Penicillium aurantiogriseum AUMC 5605 in shake flask cultivation. FTase production was optimized in two steps using DeMeo’s fractional factorial design. A 1.46-fold increase in FTase production (105.4 U/mL) was achieved using the optimized culture medium consisting of (g/L): sucrose, 600; yeast extract, 10; K2HPO4, 5; MgSO4·7H2O, 0.5; (NH4)2SO4, 1.0 and KCl, 0.5.

Moringa oleiferaLam. (Moringaceae) is widely consumed in tropical and subtropical regions for their valuable nutritional and medicinal characteristics. Recently, extensive research has been conducted on leaf extracts of M. oleifera to evaluate their potential cytotoxic effects. However, with the exception of antimicrobial and antioxidant activities, little information is present on the cytotoxic activity of the essential oil obtained from M.

Objective: Kefiran is one of the important microbial polysaccharides of many pharmaceutical and nutraceutical applications. It shows many potential medical applications as antimicrobial and immunomodulatory compound. The present work focuses on medium optimization for kefiran production using the standard strain Lactobacillus kefiranofaciens in submerged cultivation system.

Rifamycins are  broad spectrum and important antibiotics used in the treatment of  pathogenic diseases. They are generally  produced  by  the  fermentative  cultivation  of  Amycolatopsis  mediterranei,  using  submerged  cultivation