Morphometric and karyological variations were analysed within the Moorish gecko Tarentola mauritanica sensu lato from Tunisia, and compared with already published data. The results show high levels of interspecific variability, indicative of the existence of two distinct lineages: the larger one T. fascicularis, widespread in the south, nearly identical to specimens from Libya and Egypt and the other one, T. m. mauritanica, confined to the northern and central regions, belonging to the common “European” and “North African” morphotype.