Biochar (BC), a carbon-rich solid product of biomass, and its surface activation via steam have been recognized as alternative economically viable strategy to decontaminate wastewaters. Existence of glyphosate, the most extensively used non-selective herbicide, in waters at elevated concentrations has received worldwide attention due to its ill consequences. The main objective of the present study was to investigate the potential of steam activated BC produced from rice husk (RHBC) via slow pyrolysis at 700 °C to remove glyphosate from aqueous solution.