Traditional medicinal literature and previous studies have reported the possible role of Cissus quadrangularis (CQ) as an anti-osteoporotic
agent. This study examines the effectiveness of CQin promoting osteoblast differentiation of the murine pre-osteoblast cell line,MC3T3-E1.
Ethanolic extract ofCQ(CQ-E) was found to affect growth kinetics ofMC3T3-E1 cells in a dosage-dependentmanner. High concentrations of
CQ-E (more than 10mg/ml) have particularly adverse effects, while lower concentrations of 0.1 and 1mg/ml were non-toxic and did not affect