This in vitro study evaluated the microbial leakage of Cavit, IRM, and Dyract when used as temporary filling materials after root canal treatment. The degree of coronal leakage was assessed by using a microbiological marker consisting of Streptococcus faecalis and Candida albicans . For each of the two organisms, a set of 15 maxillary premolars were prepared chemomechanically and obturated with thermoplasticized gutta-percha.