To evaluate the efficacy of an ethanolic Salvadora persica extract in removing the smear layer following a root canal procedure. Methods: Sixty extracted, single-rooted human teeth were cleaned, shaped, and divided into four groups. Experimental groups 1 (n= 20) and 2 (n=20) were irrigated with 1 mg/ml and 5 mg/ml of S. persica, respectively. The positive controls (n= 10) were irrigated with 17% ethylenediaminetetraacetic acid (EDTA), while the negative controls (n= 10) were irrigated with saline.