Objectives: 1) To determine tooth size ratios in Saudi subjects with Class II, Division 1 malocclusion, 2) to compare tooth size ratios between Saudi males and females and 3) to compare tooth size ratios between Saudi subjects with Class II, Division 1, and Saudi subjects with Class I normal occlusion, and Bolton’s results. Methods: The current study consisted of study models of sixty subjects (30 males and 30 females), aged 13 to 20 years. All subjects had Class II, Division 1 malocclusion.

In the present work, we undertake the preparation of chitosan nanofibers via electrospinning by varying solution concentration (1, 2, 3, 4 and 6 wt% (w/v)), applied voltage (14, 18 and 22 kV), flow rate (0.4 mL/h and 0.8 mL/h), needle diameter (0.2 mm and 0.8 mm) and distance between needle tip and collector (100 mm and 140 mm).