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Dr. Quaiser Saquib

Professor

Professor

كلية العلوم
Building 5, 2B129 (Time: 9:00 AM- 4:00 PM)
المنشورات
مقال فى مجلة
2009

Assessment of methyl thiophanate-Cu (II) induced DNA damage in human lymphocytes

J., Saquib, Q., Al-Khedhairy, A.A., Al-Arifi, S., Dhawan, A., Musarrat, . 2009

Dimethyl 4,4′-(O-phenylene)bis(3-thioallophanate), commonly known as methyl thiophanate (MT), is a category-III acute toxicant and suspected carcinogen to humans. Hence, the ability of this benzimidazole class of fungicide to engender DNA strand breaks was investigated using alkaline single cell gel electrophoresis (SCGE), alkaline unwinding and cytokinesis-blocked micronucleus (CBMN) assays. The SCGE of human lymphocytes treated with 1 mM MT for 3 h at 37 °C showed much higher Olive tail moment (OTM) value of 40.3 ± 2.6 (p < 0.001) vis-à-vis 3.3 ± 0.09 in DMSO control. Treatment of cultured lymphocytes for 24 h resulted in significantly increased number of binucleated micronucleated (BNMN) cells with a dose dependent reduction in the nuclear division index (NDI). Stoichiometric data revealed the intrinsic property of MT to bind with Cu (II) and its reduction to Cu (I), which is known to form reactive oxygen species (ROS). We have detected the intracellular ROS generation in MT treated lymphocytes and observed an elevated level of MT-induced strand breaks per unit of calf thymus DNA in presence of Cu (II). Overall the data suggested that the formation of MT–Cu (II)–DNA ternary complex and consequent ROS generation, owing to Cu (II)/Cu (I) redox cycling in DNA proximity, is responsible for MT-induced DNA damage.

نوع عمل المنشور
KSU Research Work
رقم المجلد
23
رقم الانشاء
5
مجلة/صحيفة
Toxicology In Vitro
الصفحات
848–854
مزيد من المنشورات
publications

Tris(1,3-Dichloro-2-propyl)phosphate (TDCPP) is an organophosphorus flame retardant (OPFR) widely used in a variety of consumer products (plastics, furniture, paints, foams, and electronics).

بواسطة Quaiser Saquib, Abdullah M. Al-Salem, Maqsood A. Siddiqui, Sabiha M. Ansari, Xiaowei Zhang, Abdulaziz A. Al-Khedhairy
2022
publications

Tris (2-ethylhexyl) phosphate (TEHP) is an organophosphate flame retardant (OPFRs) which is extensively used as a plasticizer and has been detected in human body fluids.

بواسطة Quaiser Saquib, Abdullah M. Al-Salem, Maqsood A. Siddiqui, Sabiha M. Ansari, Xiaowei Zhang, Abdulaziz A. Al-Khedhairy
2022
publications

Recent reports have confirmed that tris(2-butoxyethyl) phosphate (TBEP), an organophosphorous flame retardants (OPFRs), profoundly detected in the dust from solid waste (SW), e-waste dumping sites…

بواسطة Quaiser Saquib, Abdullah M. Al-Salem, Maqsood A. Siddiqui, Sabiha M. Ansari, Xiaowei Zhang, Abdulaziz A. Al-Khedhairy
2022