Zinc oxide Quantum Dots: Multifunctional candidates for arresting the C2C12 cancer cells and their role towards Caspase 3 and 7 genes
Ba, Rizwan Wahab, Farheen Khan, You bing Yang, I.H. Hwang, Hyung-Shik Shin, Javed Ahmad, Sourabh Dwivedi, Shams T. Khan, Maqsood Ahmed Siddiqui, Quaiser Saquib, Javed Musarrat, Abdulaziz A. Al-Khedhairy, Yogendra K. Mishra . 2016
Recently, nanoscale (<100 nm) inorganic materials, especially spherical shaped zinc oxide (ZnO-QDs) have received significant attention from broad community because of their potential utalizations in various technologies. Due to their large surface to volume (S/V) ratio contributions and extremely high reactivities, they can easily penetrate in various biological identities, like cells, proteins and hence can sense, diagnose and cure different biological systems. The present study describes the simple synthesis of crystalline ZnO-QDs via solution process and C2C12 myoblast cancer cells have been treated with different doses of ZnO-QDs at diffrent incubation (24, 48, 72 and 96 h) time. The rate of inhibition of cells was observed with MTT assay whereas morphology of cells was observed via confocal microscopy (CLSM). The MTT and CLSM investigations confirmed that with increase in the incubation time, the population density of cancer cells was decreased when treated with ZnO-QDs. The dose dependent apoptosis correlated intracellular production of reactive oxygen species (ROS) from C2C12 cancer cells was also measured in presence of ZnO-QDs. Apart from this, the effect/apoptosis of these QDs were also checked in presence of candidate genes such as caspase 3/7 with GAPDH. The Reverse transcription polymerase chain reaction (RT-PCR) analysis demonstrates the up-regulation of caspase 3/7 genes in cells subsequently treated with ZnO-QDs at low and high concentrations.
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