CLS 331
CLS 331 Clinical Biochemistry II (3+1=4)
Course work involves a study of the metabolic activity of animal tissue and its regulation. Major catabolic pathways related to carbohydrate, amino acid, and fatty acids are discussed in detail. The study includes a look at the bio-energetic of cells as well as the digestion and absorption of food material.
كلن 331 كيمياء حيوية إكلينيكية ( 2 ) ( 3 + 1 )
يهتم هذا المقرر بوصف لعمليات الإيض الخلوية الخاصة بأكسدة كل من السكاكر والأحماض الأمينية والأحماض الدهنية وطرق استخراج الطاقة الكيميائية منها وتخزينها كما يشمل دراسة عن دور الهرمونات المنظمة لعمليات الإيض وكذلك العيوب الخلقية المتسببة عن نقص انزيمات الأيض ويشمل المقرر ايضا على وصف لعملية الهضم وامتصاص المواد الغذاذية.
Course Outline:
CLS 331 Laboratory Schedule
Week subject
1 . The hydrolytic activity of salivary amylases on starch .
2 . Quantitative determination of amylases activity .
3 . The production of pyruvate and acetaldehyde during the fermentation
of glucose by yeast .
4 . Identification of free amino acids by TLC using silica gel plates.
5 . Quantitative determination of protein by the biuret and Lowry Reactions.
6 . Enzymatic Digesiton of fat by pancreatic lipase .
7 . Effect of Bile salts on pancreatic lipase activity .
8 . Determination of serum urea by the berthelot reaction .
9 . The determination of the phosphorus content of a nucleic acid .
10 . Determination of inorganic phosphate .
11 . Determination of the glycogen content in liver homogenate using the
An throne Reaction .
12 . Revision
13 . FINAL LABORATORY EXAMINATION .
Exam Model:
Q: Describe briefly the principle of glucose oxidation?
Q: What is starch ?
Q: Describe briefly the uses of biuret method?
Refrences:
CLS 331 Practical booklet
Course Materials