Anti-biofilm and antibacterial activities of zinc oxide nanoparticles against the oral opportunistic pathogens Rothia dentocariosa and Rothia mucilaginosa.

Journal Article

, khan ST, Ahamed M, Musarrat J, Al-Khedairy AA. . 2014

• Publication Online URL: Anti-biofilm and antibacterial activities of zinc oxide nanoparticles against the oral opportunistic pathogens Rothia dentocario
• Magazine \ Newspaper: European Journal of Oral Sciences
• Volume Number: 122
• Pages: 397–403

Publication Abstract

Species of the genus Rothia that inhabit the oral cavity have recently been implicated
in a number of diseases. To minimize their role in oral infections, it is imperative
to reduce and/or control the growth and biofilm formation activity of Rothia
spp. In this study, two bacterial isolates, Ora-7 and Ora-16, were obtained from the
oral cavity of a healthy male subject and identified as Rothia dentocariosa and
Rothia mucilaginosa, respectively, using a polyphasic taxonomic approach. Antimicrobial
and anti-biofilm formation activities of zinc oxide nanoparticles (ZnO-NPs),
of average size 35 nm, were assessed in in vitro assays using Crystal Violet and live
and dead staining techniques. The ZnO-NPs exhibited an IC50 value of 53 and
76 µg ml-1 against R. dentocariosa and R. mucilaginosa, respectively. Biofilmformation
assays, performed on the surfaces of polystyrene plates, artificial teeth,
and dental prostheses, revealed the efficacy of ZnO-NPs as a potential antibacterial
agent for controlling the growth of Rothia isolates in both planktonic form and
biofilm.