Dr. Seema Zargar
Postal Address: Phone: 8051692
E-mail: seemazargar@yahoo.com, szargar@ksu.edu.sa
Department of Biochemistry
College of science, King Saud university
Prince Turkey Road
Riyadh; Saudi Arabia-11495
PROFESSIONAL QUALIFICATIONS: Ph.D. Biotechnology under supervision of Prof. S. K. Jain, Dept. of Biotechnology, Hamdard University, New Delhi-62.
RESEARCH TOPIC of PhD:
Molecular mechanism of pathogenesis of dengue virus and its interaction with host cell proteins.
Purification of recombinant envelope protein of Dengue virus (infective domain) was done by Ni-NTA affinity column and it was labeled and interacted with blotted two dimensional gels of total proteins of mammalian cells. This interaction could show us the proteins that might be responsible for DEN-virus entry or its propagation in mammalian cells. The principal techniques used in the study were Isolation of total proteins, one dimensional gel electrophoresis, two dimensional gel electrophoresis, far Western blotting, Ni-NTA protein purification of expressed proteins, electron microscopy and MALDI-TOF analysis, etc
WORK EXPERIENCE:
2016-till date: Associate Professor at King Saud University. Riyadh.
2011-2016: Assistant Professor at King Saud University. Riyadh.
2009-2011: Associate Scientist at King Faisal Hospital and Research Centre, Riyadh.
2003-2009: PhD Biotechnology, Jamia Hamdard and teaching Assistant.
EXPERTISE DEVELOPED DURING Post doctorate
FACS analysis of CD3, CD4 and CD8, CD25, CD44 and Ki67 antibodies.
MACS separation of different cell types (Magnetic cell sorting)
Adipose tissue separation and culturing from lipoaspirate of abdominal fat or gluteal fat tissue
Elisa for IL-6, adiponectin, Insulin, leptin,TNF-a and other adipokines.
Isolation of thymocytes and splenocytes from mice and their culture.
Extraction of DNA from mouse tail and their genotypic.
Extraction of protein and Western blotting of cell lysates.
Culturing of different types of primary and secondary cultures including established cell lines.
Gene expression by Reverse transcription PCR.
Cloning, cDNA library preparation and yeast two hybrid selection, Real time PCR, tunnel assay and double immunofluorescence.
Supervising Trainees and students from other institutes like King Saud University, Alfaisal University, College of Pharmacy and Dentistry, etc.
TEACHING EXPERIENCE
Topics taught to undergraduate science studies include: Biomembranes, Biochemistry of specialized tissues and genetic engineering and biotechnology, molecular carcinogenesis, Antibiotics, Immunology, Gene expression. Topics taught to postgraduate students of Biochemistry include Molecular biology and protein targeting and selected Topics in Biochemistry.
Metabolism and molecular biology in Jamia Hamdard.
SUPERVISOR OF FOLLOWING THESES
1. Effect of expression of STK11 and its promoter activity in human breast cancer cell lines. (PI)
2. Polymorphic pattern of certain genes in ovarian cancer in Saudi patients. (Co-PI)
3. Innate immunity and ovarian cancer: Role of the inflammatory cytokines (IL-1b and TNF-a) and their receptors (IL-1 receptor and TNF receptor). (Co-PI)
4. Anti-microbial activity of zingier officinal and curcum longa extracts against some selected micro-organisms’. (PI)
5. Efficacy of medicinal plant extracts in growth of fungi and subsequent production of citrinin(PI) (selected in Scientific excellence for King Saud university students)
6. Assessment various impurities of water from several well water supplies from Riyadh region of Saudi Arabia (PI) .
7. Efficacy of date extract on inhibition of Citrinin production by Penicillium notatum and Aspergillus flavus (PI).
8. Cloning and sequencing of camel SOD gene (co-PI)
Other Activities
Member, Postgraduate Committee 2014-2017
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
Member, Accreditation Committee 2016-till date
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
Member, Laboratory Committee 2011-2013
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
Member, Examination Committee 2011-2014
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
Member, Safety Committee 2011-2013
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
ACHIEVEMENTS:
Scored second position in M. Sc Biochemistry
Qualified JRF and Eligibility for Lectureship (NET) held on 22nd June 2003 conducted by Council of Scientific and Industrial Research, New Delhi (All India Level Exam).
Senior Research Fellowship from Council of Scientific and Industrial Research (All India Level Eligibility Test).
Qualified GATE 2004 with 94.5 percentile at All India Level.
LIST OF PUBLICATIONS:
CONFERENCES AND WORKSHOPS:
REVIEWER
Reviewer to Journal of Infectious disease and immunity, Intervirology, Indian journal of Biological Sciences, Hindawi publishing systems,Science international
MEMBERSHIPS:
Member of European Federation of Biotechnology.
Member of Indian Immunological society.
Society of cancer research
EDUCATIONAL QUALIFICATIONS:
1999: B.Sc. Kashmir University, Hazratbal Srinagar
2003: M.Sc Jamia Hamdard University, Delhi
2009: Ph. D, Jamia Hamdard University, Delhi
Attended a computer literacy programme conducted by the Department of Electronics and Communication Engineering of REC, Srinagar during May- July, 2000. Ample knowledge of computers with command on Windows, MS Office and internet world
Gender : Female
Marital Status : Married
Hobbies : Travelling, & Internet surfing.
Nationality : Indian
Permanent Address : House No.29, New Housing Colony, Butta Kadal, Lal Bazar , Srinagar , Kashmir, India -190006
Seema Zargar
Postal Address: Phone: 8051692
E-mail: seemazargar@yahoo.com, szargar@ksu.edu.sa
Department of Biochemistry
College of science, King Saud university
Prince Turkey Road
Riyadh; Saudi Arabia-11495
PROFESSIONAL QUALIFICATIONS: Ph.D. Biotechnology under supervision of Prof. S. K. Jain, Dept. of Biotechnology, Hamdard University, New Delhi-62.
RESEARCH TOPIC of PhD:
Molecular mechanism of pathogenesis of dengue virus and its interaction with host cell proteins.
SUMMARY OF THESIS
Purification of recombinant envelope protein of Dengue virus (infective domain) was done by Ni-NTA affinity column and it was labeled and interacted with blotted two dimensional gels of total proteins of mammalian cells. This interaction could show us the proteins that might be responsible for DEN-virus entry or its propagation in mammalian cells. The principal techniques used in the study were Isolation of total proteins, one dimensional gel electrophoresis, two dimensional gel electrophoresis, far Western blotting, Ni-NTA protein purification of expressed proteins, electron microscopy and MALDI-TOF analysis, etc
WORK EXPERIENCE:
2016-till date: Associate Professor at King Saud University. Riyadh.
2011-2016: Assistant Professor at King Saud University. Riyadh.
2009-2011: Associate Scientist at King Faisal Hospital and Research Centre, Riyadh.
2003-2009: PhD Biotechnology, Jamia Hamdard and teaching Assistant.
EXPERTISE DEVELOPED DURING Post doctorate
FACS analysis of CD3, CD4 and CD8, CD25, CD44 and Ki67 antibodies.
MACS separation of different cell types (Magnetic cell sorting)
Adipose tissue separation and culturing from lipoaspirate of abdominal fat or gluteal fat tissue
Elisa for IL-6, adiponectin, Insulin, leptin,TNF-a and other adipokines.
Isolation of thymocytes and splenocytes from mice and their culture.
Extraction of DNA from mouse tail and their genotypic.
Extraction of protein and Western blotting of cell lysates.
Culturing of different types of primary and secondary cultures including established cell lines.
Gene expression by Reverse transcription PCR.
Cloning, cDNA library preparation and yeast two hybrid selection, Real time PCR, tunnel assay and double immunofluorescence.
Supervising Trainees and students from other institutes like King Saud University, Alfaisal University, College of Pharmacy and Dentistry, etc.
TEACHING EXPERIENCE
Topics taught to undergraduate science studies include: Biomembranes, Biochemistry of specialized tissues and genetic engineering and biotechnology, molecular carcinogenesis, Antibiotics, Immunology, Gene expression. Topics taught to postgraduate students of Biochemistry include Molecular biology and protein targeting and selected Topics in Biochemistry.
Metabolism and molecular biology in Jamia Hamdard.
SUPERVISOR OF FOLLOWING THESES
1. Effect of expression of STK11 and its promoter activity in human breast cancer cell lines. (PI)
2. Polymorphic pattern of certain genes in ovarian cancer in Saudi patients. (Co-PI)
3. Innate immunity and ovarian cancer: Role of the inflammatory cytokines (IL-1b and TNF-a) and their receptors (IL-1 receptor and TNF receptor). (Co-PI)
4. Anti-microbial activity of zingier officinal and curcum longa extracts against some selected micro-organisms’. (PI)
5. Efficacy of medicinal plant extracts in growth of fungi and subsequent production of citrinin(PI) (selected in Scientific excellence for King Saud university students)
6. Assessment various impurities of water from several well water supplies from Riyadh region of Saudi Arabia (PI) .
7. Efficacy of date extract on inhibition of Citrinin production by Penicillium notatum and Aspergillus flavus (PI).
8. Cloning and sequencing of camel SOD gene (co-PI)
Other Activities
Member, Postgraduate Committee 2014-2017
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
Member, Accreditation Committee 2016-till date
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
Member, Laboratory Committee 2011-2013
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
Member, Examination Committee 2011-2014
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
Member, Safety Committee 2011-2013
Department of Biochemistry, College of Science,
Sciences & Medical Studies Department for Women Students,
King Saud University, Riyadh, Saudi Arabia.
ACHIEVEMENTS:
Scored second position in M. Sc Biochemistry
Qualified JRF and Eligibility for Lectureship (NET) held on 22nd June 2003 conducted by Council of Scientific and Industrial Research, New Delhi (All India Level Exam).
Senior Research Fellowship from Council of Scientific and Industrial Research (All India Level Eligibility Test).
Qualified GATE 2004 with 94.5 percentile at All India Level.
LIST OF PUBLICATIONS:
- Asma Alkaf, Abdulaziz Al-Jafari, T A. Wani, S Alqattan, Seema Zargar (2017). Expression of STK11 gene and its promoter activity in MCF control and cancer cells. 3 Biotech 7:362. DOI 10.1007/s13205-017-1000-6.
- Tanveer A Wani*, Seema Zargar, Mohammed A. Hamidaddin,Ibrahim A. Darwish. Spectrophotometric and molecular modelling studies on in vitro interaction of tyrosine kinase inhibitor linifanib with bovine serum albumin. Plosone. Published:April18,2017. https://doi.org/10.1371/journal.pone.0176015.
- T A. Wani , AH. Bakheit , A Al-Majed, MA Bhat and Seema Zargar. Study of the Interactions of Bovine Serum Albumin with the New Anti-Inflammatory Agent 4-(1,3-Dioxo-1,3-dihydro-2H-isoindol-2-yl)-N′-[(4-ethoxy-phenyl)methylidene]benzohydrazide Using a Multi-Spectroscopic Approach and Molecular Docking. Molecules 2017, 22(8),1258; doi:10.3390/molecules22081258.
- Seema Zargar, T A. Wani, A A Alamro, MA Ganaie. (2017). Amelioration of Thioacetamide Induced Liver Toxicity in Wistar Rats by Rutin.
- Seema Zargar , N J Siddiqi, S Ansar, M Alsulaimani & A. El ansary (2016). Therapeutic role of quercetin on oxidative damage induced by acrylamide in rat brain. Pharmaceutical Biology.
2. T A. Wani and Seema Zargar (2015). New ultra-performance liquid chromatographytandem mass spectrometry method for the determination of irbesartan in human plasma. Journal of food and drug analysis. 2 3; 5 6 9 -5 7 6.
3. Seema Zargar, T. A. Wani, S. K. Jain. (2015). Identification of Putative Vero Cell Protein(s) that Bind Specifically to Recombinant Envelope Protein of Dengue Virus Type 2. Tropical journal of Pharmaceutical Research. 14,(6) 997-1003.
4. T A. Wani , A.M. Alanazi, Seema Zargar, A. Ahmad, I. Darwish (2015). Box–Behnken Design Based Statistical Modelling for Optimization of UPLC-MS/MS Method for Analysis of Sorafenib in Bulk and Tablets. Journal of Computational and Theoretical Nanoscience. 12, 3598–3604.
5. T A. Wani and Seema Zargar (2015). New Highly-Sensitive Ultra-Performance Liquid Chromatography - Mass Spectrometry Method for Quantification of Telmisartan in Human Plasma. TJPR 14 (3): 511-518.
6. Seema Zargar, N JSiddiqi, S Al Daihan, TA. Wani. (2015) Protective effects of quercetin on cadmium fluoride induced oxidative stress at different intervals of time in mouse liver. Acta biochimica polonica 62(2) 207-213.
7. Tanveer A. Wani, Seema Zargar and Ajaz Ahmad. Ultra Performance Liquid Chromatography Tandem Mass Spectrometric Method Development and Validation for Determination of Neratinib in Human Plasma. S. Afr. J. Chem., 2015, 68, 93–98
8. N JSiddiqi and Seema Zargar (2015).. Quercetin protects mice kidney collagen against the damage induced by cadmium fluoride. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.
9.TA Wani and Seema Zargar. (2014).Sensitive UPLC-tandem mass spectrometric method for determination and validation of hydrochlorthiazide in human plasma. Lat. Am. J. Pharm, accepted on nov 21/ 2014.
10. Seema Zargar. (2014). Inhibitory effect of various aqueous medicinal plant extracts production and fungal biomass by Penicillium notatum and Aspergillus niger. IAIM. 1(3): 1-8.
11. Seema Zargar, NJ Siddiqi, TH Khan, IE Elredah. (2014). Effect of cadmium fluoride and quercetin on in vivo activity of indoleamine 2,3-dioxygenase in mice liver and kidney. Fluoride. 47(1): 31–42. - 12.NJ Siddiqi and Seema Zargar (2014). Effect of quercetin on cadmium fluoride induced alterations in hydroxyproline/collagen content in mice liver. Connective tissue research. 55(3): 234-238.
- 13. Seema Zargar and TA Wani. (2014). Ultra-performance liquid chromatography/tandem mass spectrometry method for simultaneous determination of irbesartan and hydrochlorthiazide in human plasma. Accepted in Journal of the Iranian Chemical Society. DOI 10.1007/s13738-014-0429-3
- 14. Seema Zargar and T A. Wani. (2014). New UPLC-MS/MS method for simultaneous determination of telmisartan and hydrochlorthiazide in human plasma. Lat. Am. J. Pharm, 33(3);432-40.
- 15. Seema zargar. (2014). Protective effect of Trigonella foenum-graecum on thioacetamide induced hepatotoxicity in rats. Saudi Journal of Biological Sciences.
- 16.TA Wani and Seema Zargar (2015). Statistical modelling for optimization of automated spectrophotometric assay for determination of cefadroxil in its pharmaceutical formulations. Accepted in Lat. Am. J. Pharm
- 17. Tanveer A. Wani, Muzaffar Iqbal, Ibrahim Darwish Nasr Y. Khalil, Seema Zargar.(2014) Development and validation of sensitive uplc-ms/ms based method for the estimation of crizotinib in human plasma. Digest Journal of Nanomaterials and Biostructures. 9:693-704.
- 18. Perwez Alam, Mohammad S. Ahmad, Tanveer Ahmad Wani, Seema Zargar and Nasir A. Siddiqui.(2014). Simultaneous quantification of two bioactive flavonoids Homoeriodictyol and Persicogenin in the methanol extract of aerial parts of two different species of genus Rhus by validated HPTLC densitometric method. Accepted in Journal of Planar Chromatography - Modern TLC October 2014. Manuscript No. JPC528R1
- 19. Seema zargar, S. wakil, A. mobeirek, A. al‑jafari. (2013). Involvement of ATP‑binding cassette, subfamily A polymorphism with susceptibility to coronary artery disease. Biomedical Reports:, 883-888.
- 20. Darwish I A., Wani TA., Alanazi AM., Hamidaddin MA., Seema Zargar. (2013) Kinetic-exclusion analysis-based immunosensors versus enzyme-linked immunosorbent assays for measurement of cancer markers in biological specimens. Talanta. 111, 13-19.
- 21. S. Al-Daihan, M. Al-Faham, N. Al-shawi, R. Almayman, A. Brnawi, Seema zargar R. S. Bhat. (2013). Anti-microbial activity of zingier officinal and curcum longa extracts against some selected micro-organisms. Journal of King Saud University - Science, April, Volume 25, Issue 2, Pages 115-120.
- 22. T. A. Wani, A. Ahmad, Seema Zargar, Nasr Y. Khalil, Ibrahim A. (2012). Darwish. Use of response surface methodology for optimization and validation of new microwell-based spectrophotometric method for determination of atorvastatin calcium in tablets. Chemistry Central Journal., 6:134 doi:10.1186/1752-153X-6-134.
- 23. Seema Zargar, Tanveer A. Wani, S. K. Jain. (2011). Morphological changes in vero cells postinfection with dengue virus type-2. Microscopy Research and Techniques. 74, 314-319.
- 24. T. A. Wani, M. Al-Omar, Seema Zargar. Huntington Disease: Current A dvances in Pathogenesis and Recent Therapeutic Strategies. International Journal of Pharmaceutical Sciences and Drug Research. 2011; 3(2): 69-79.
- 25. Seema zargar and S. K. Jain. (2008). Published several sequence in Uniprot KB/Swiss-Prot entry under accession no. p84856, p694580.
- 26. Seema and S.K.Jain, (2005). Molecular mechanism of pathogenesis of dengue virus: Entry and fusion with target cell. Ind. Jour. Clin. Biochem., 20 (2) 92-103.
CONFERENCES AND WORKSHOPS:
- Seema and S.K. Jain. Proteomic approach for characterization of cell surface receptors for dengue virus: protein profile of permissive and non-permissive cells. Biotechnology and Human welfare magazine. 2006. Hamdard University. New Delhi.
- Seema and Jain S. K. proteome profile of permissive and non-permissive cells for different serotypes of dengue virus. 33rd immunological society proceedings. 2004, 341-342.
- Attended Interdisciplinary Science Conference on Recent Trends in Research in Biological Sciences.
- Attended National Conference on Electron Microscopy and Allied Fields and XXIX Annual Meeting of EMSI.
REVIEWER
Reviewer to Journal of Infectious disease and immunity, Intervirology, Indian journal of Biological Sciences, Hindawi publishing systems,Science international
MEMBERSHIPS:
Member of European Federation of Biotechnology.
Member of Indian Immunological society.
Society of cancer research
EDUCATIONAL QUALIFICATIONS:
1999: B.Sc. Kashmir University, Hazratbal Srinagar
2003: M.Sc Jamia Hamdard University, Delhi
2009: Ph. D, Jamia Hamdard University, Delhi
PERSONAL DETAILS:
Attended a computer literacy programme conducted by the Department of Electronics and Communication Engineering of REC, Srinagar during May- July, 2000. Ample knowledge of computers with command on Windows, MS Office and internet world
Gender : Female
Marital Status : Married
Hobbies : Travelling, & Internet surfing.
Nationality : Indian
Permanent Address : House No.29, New Housing Colony, Butta Kadal, Lal Bazar , Srinagar , Kashmir, India -190006
Seema Zargar
Course deals with structure and functioning of membranes
Lehninger,stryr and Harpers biochemistry should be consulted
The course deals mostly with human genetics mostly the book consulted is Hartwell genetics
The course is divided into three modules: 4 weeks per module. A research paper will be assigned to be presented by the student in week 13 and 14.Lewin’s Gene X is the reference book