FISH SCALES - A NON-INVASIVE ASSESSMENT TOOL FOR EVALUATING THE EFFECT OF POLLUTION ON CIRRHINUS MRIGALA FROM THE RAVI RIVER, PAKISTAN

Journal Article
Z., Mahboob, S., Al-Ghanim, K. A., Sultana, S., Alkahem Al- Balawi, H. F., Sultana, T., Ashraf, A., Al- Misned, F. and Ahmed, . 2015
Publication Work Type: 
Research Article
Magazine \ Newspaper: 
The Journal of Animal & Plant Sciences
Issue Number: 
4
Volume Number: 
25
Pages: 
1135-1139
Publication Abstract: 

The present study evaluated the effects of water pollution on Cirrhinus mrigala in the Ravi River by comparing DNA
extracted non-invasively from their scales to DNA extracted from the scales of fish collected from controlled fish farm.
A single, random sampling was conducted. Fish were broadly categorised into three weight categories: W1 (500 to 1000
g), W2 (1001 to 1500 g) and W3 (1501 to 2000 g). DNA was extracted non-invasively from control and experimental
samples. The quantity and quality of DNA from the control and experimental samples were compared. The experimental
samples in the W1, W2 and W3 categories had an average DNA concentration (μg/μl) that was lower than the control
samples. All control samples had a single DNA band; whereas the experimental samples in W1 fish had 1 to 2 bands, the
experimental samples in W2 fish had two bands and the experimental samples in W3 fish had fragmentation in the form
of three bands. These bands show the effects of pollution on fish in the Ravi River. We concluded that this non-invasive
assessment tool could be successfully used in scale-bearing fish species for assessment of contaminants and damage in
the DNA as a rapid, non-lethal and biologically reliable indicator of water quality for the presence of various toxicants in
surface water and their effect on the fish health.