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Amal Aldarwesh

Assistant Professor

Faculty member

كلية العلوم الطبية التطبيقية
Prince Turki Ibn Abdul Aziz Al Awwal Road, Riyadh 12371, Saudi Arabia
المنشورات
مقال فى مجلة
2010

Carnitine Deficiency Aggravates Cyclophosphamide-Induced cardiotoxicity in Rats

Fatani, Amal J. . 2010

BACKGROUND:

This study examined, for the first time, the involvement of carnitine deficiency in cardiotoxicity, particularly cyclophosphamide (CP)-induced cardiomyopathy, as well as effects of carnitine supplementation with propionyl-L-carnitine (PLC) on cardiotoxicity.

METHODS:

An animal model of carnitine deficiency was developed in rats treated with D-carnitine (DC)-mildronate (MD). Adult male Wistar albino rats were assigned to one of six treatment groups: the first three groups were injected intraperitoneally with normal saline, PLC (250 mg/kg/day), and DC (250 mg/kg/day) combined with MD (200 mg/kg/day), respectively, for 10 successive days. In groups 4-6, the same doses of normal saline, PLC and DC-MD were injected, respectively, during the 5 successive days before and after a single dose of CP (200 mg/kg). On day 6 after CP treatment, 24-hour urine was collected, then animals were sacrificed, and serum as well as hearts were isolated.

RESULTS:

CP caused a significant increase in serum creatine phosphokinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), urinary carnitine excretion and clearance and intramitochondrial acetyl-CoA/CoA-SH, and a significant decrease in serum free carnitine, total carnitine and adenosine triphosphate (ATP) contents in cardiac tissue. In the carnitine-depleted rats, CP induced dramatic increases in CK-MB and LDH levels, carnitine clearance and intramitochondrial acetyl-CoA/CoA-SH, as well as progressive reduction in total carnitine and ATP in cardiac tissues. Interestingly, PLC supplementation completely reversed the biochemical and histopathological changes induced by CP to the control values.

CONCLUSION:

(1) Carnitine deficiency is a risk factor which is involved in CP-related cardiomyopathy; (2) serum and urinary carnitine levels should be monitored and viewed as indices of CP-induced multiple organ toxicity, and (3) carnitine supplementation, using PLC, prevents the development of CP-induced cardiotoxicity.

 

نوع عمل المنشور
Master
رقم المجلد
56
مجلة/صحيفة
Chemotherapy
الصفحات
71-81
مزيد من المنشورات
publications

PURPOSE:

Elevated intraocular pressure (IOP) is a major risk factor for glaucoma. One consequence of raised IOP is that ocular tissues are subjected to increased hydrostatic pressure (HP).…

بواسطة Andrew Osborne, Amal Aldarwesh, Jeremy Rhodes, Peter Sidaway, David Broadway, Claire Everitt, Julie Sanderson
2015
publications

Education in the ethical conduct of research that is acquired by postgraduate students is a key component

for their professional development in scientifi c and academic communities. This…

بواسطة Amal Q.M. Aldarwesh
2013
publications

This study compared efficacy of two polyvalent antivenoms (Saudi Arabian and Egyptian), against lethality and pathophysiological changes of Leiurusquinquestriatus quinquestriatus (LQQ) scorpion …

بواسطة Amal J. Fatani, Amany A.E. Ahmed, Rabab M. Abdel-Halim, Mary Martin, Nuzha A. Abdoon, Amal Q. Aldarwesh
2010