Effect of battery-manufacturing effluent on endogenous antioxidant in freshwater fish

Journal Article
, Anwar Ahmad & Daoud Ali . 2013
Magazine \ Newspaper: 
Chemical Speciation & Bioavailability - Taylor & Francis
Issue Number: 
Volume Number: 
Publication Abstract: 

This study monitors and assesses the effect of battery-manufacturing effluent containing metals Pb, Zn and Cd
on endogenous antioxidants. Malonaldehyde (MDA), reduced glutathione sulfyhydryl (GSH) and catalase (CAT)
which are known biomarkers of effluent were exposed to 0%, 25%, 50%, 75% and 90% amendments for 74h on
the gills, liver and kidneys of C. punctata. There was more metal Zn accumulation in the gills and GSH contents
increased significantly in the gills (P<0.01), liver accumulation of Pb was found to be more (P<0.05), whereas lowest
accumulation of Pb was found in kidneys and the highest accumulation of Cd (P<0.05). Over all amendments of the
effluents, MDA contents were increased in the gills, liver and kidneys (P<0.01). GSH levels were decreased among
the liver and kidneys compared to the gills (P<0.01) at 90% amendment. Effluent exposure caused a significant
decrease in the activities of CAT in the gills, liver and kidneys (P<0.01, 0.05 and 0.05) of fish. Increased MDA activity
was indicative of the formation of free radicals in the fish with exposure to amendments of battery manufacturing
effluent, while increased levels of GSH pointed to the occurrence of a scavenging mechanism of free radicals.