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Dr Ali Daoud

Professor

Faculty

كلية العلوم
Department of Zoology College of Science King Saud University, Riyadh, Saudi Arabia
المنشورات
مقال فى مجلة
2015

Quantum Dot-Based Molecular Beacon to Monitor Intracellular MicroRNAs

, Jonghwan Lee, Sung Ung Moon, Yong Seung Lee, Bahy A. Ali, Abdulaziz A. Al-Khedhairy, Daoud Ali . 2015

Fluorescence monitoring of endogenous microRNA (miRNA or miR) activity related to neuronal development using nano-sized materials provides crucial information on miRNA expression patterns in a noninvasive manner. In this study, we report a new method to monitor intracellular miRNA124a using quantum dot-based molecular beacon (R9-QD-miR124a beacon). The R9-QD-miR124a beacon was constructed using QDs and two probes, miR124a-targeting oligomer and arginine rich cell-penetrating peptide (R9 peptide). The miR124a-targeting oligomer contains a miR124a binging sequence and a black hole quencher 1 (BHQ1). In the absence of target miR124a, the R9-QD-miR124a beacon forms a partial duplex beacon and remained in quenched state because the BHQ1 quenches the fluorescence signal of the R9-QD-miR124a beacon. The binding of miR124a to the miR124a binding sequence of the miR124a-targeting oligomer triggered the separation of the BHQ1 quencher and subsequent signal-on of a red fluorescence signal. Moreover, enhanced cellular uptake was achieved by conjugation with the R9 peptide, which resulted in increased fluorescent signal of the R9-QD-miR124a beacons in P19 cells during neurogenesis due to the endogenous expression of miR124a. 

رقم المجلد
15
مجلة/صحيفة
Sensors - An Open Access Journal from MDPI - MDPI.com
الصفحات
12872-12883
مزيد من المنشورات
publications

Metal nanomaterials such as bismuth oxide nanoparticles (Bi2O3NPs) have been extensively used in cosmetics, dental materials,pulp capping, and biomedical imaging. There is little knowledge about…

بواسطة Ali Alamer1 & Daoud Ali2 & Saud Alarifi2 & Abdullah Alkahtane2 & Mohammed AL-Zharani3 & Mohamed M. Abdel-Daim2,4 & Gadah Albasher2 & Rafa Almeer2 & Nouf K. Al-Sultan2 & Abdulaziz Almalik1,5 & Ali H Alhasan1,5 & Saa
2021
publications

Silver nanoparticles (AgNPs) possess properties that are important for industrial and medical applications. This study is aimed to investigate intra-peritoneal toxicity of AgNPs at 26, 52 or 78 mg…

بواسطة Saud Alarifi, Daoud Ali, Mohammed A Al Gurabi & Saad Alkahtani
2017
publications

The present work was designed to investigate the effect of palladium nanoparticles (PdNPs) on human skin malignant melanoma (A375) cells, for example, induction of apoptosis, cytotoxicity, and DNA…

بواسطة Saud Alarifi, Daoud Ali, Saad Alkahtani, and Rafa S. Almeer
2017