MECHANISM OF CAPSEBON NANOPARTICLES INDUCED OXIDATIVE STRESS AND APOPTOSIS IN CACO-2 CELLS
Alkahtani2, Mohammed S Aleissa1 , Daoud Ali2 , Saud Alarifi2 , Saad . 2019
Capsebon (CdS) nanoparticles are extensively used in excipients, drugs and paints, plastics, textiles, ceramic and glass industry. The high exposure to capsebon nanoparticles causes adverse effect on skin, eyes, respiratory track and kidney. The toxicity profile of capsebon nanoparticles on human colon cancer (Caco-2) cells is not finally understood. Hence, in this study capsebon nanoparticles were characterized were characterized by UV-visible spectrophotometer, dynamic light scattering and transmission electron microscope. The cell toxicity of capsebon nanoparticles was observed in Caco-2 cells by using NRU and MTT tests. The percentage of cell death was increased as the dose and time of capsebon nanoparticles exposure was increased. The hike in the production of reactive oxygen species (ROS) in Caco-2 cells due to capsebon nanoparticles was checked by applying fluorescent dye DCFDA and production of ROS was time and dose dependent. The toxicity of capsebon nanoparticles in Caco-2 cells was correlated with the generation of ROS, compromisation of mitochondrial trans membrane potential (MTP) and nucleic acid damage. Thus the current observation was confirmed that mitochondria play an important role in genotoxicity and cytotoxicity induced capsebon nanoparticles in Caco-2 cells. Also, we have examined the oxidative stress biomarkers lipid peroxide (LPO), and glutathione (GSH) and LPO was increased and GSH was decreased in Caco-2 cells. In this experiment, we noticed that capsebon nanoparticles are more dangerous at higher dose and increased time. This work deals the dose and dependent cell toxicity and apoptosis in Caco-2 cells KEYWORDS: Capsebon nanoparticles; Caco-2 cells; Oxidative stress; Cytotoxicity
Metal nanomaterials such as bismuth oxide nanoparticles (Bi2O3NPs) have been extensively used in cosmetics, dental materials,pulp capping, and biomedical imaging. There is little knowledge about…
Silver nanoparticles (AgNPs) possess properties that are important for industrial and medical applications. This study is aimed to investigate intra-peritoneal toxicity of AgNPs at 26, 52 or 78 mg…
The present work was designed to investigate the effect of palladium nanoparticles (PdNPs) on human skin malignant melanoma (A375) cells, for example, induction of apoptosis, cytotoxicity, and DNA…