ISOLATION AND IDENTIFICATION OF PATHOGENIC BACTERIA FROM DISEASED FRESHWATER FISH CATLA CATLA (HAM.)

Journal Article
Tiwari4, Divya Sahni1, Daoud Ali2,*, Ali Al-Amer3, Neeraj Sood1, D.K Chaudhary1, Ashish . 2017
Magazine \ Newspaper: 
Fresenius Environmental Bulletin
Issue Number: 
1a
Volume Number: 
26
Pages: 
1108-1115
Publication Abstract: 

Diagnosis is one of the most important steps for prevention of disease. In the present study, we haveisolated bacteria from the muscles of theabdominalinfected area of diseased fishCatlacatla, whichis thefastest growing Indian major carp and an importantfreshwater fish in Asian countries. On the basis ofprimary and secondary biochemical test, it wasconfirmed that Edwardsilatarda,Aeromonashydrophila and Vibrio cholera arefrequently present. DNA was isolated and amplifiedwith help of PCR and then RFLP was performed,which showed all the three bacterial isolates werepathogenic in nature on the basis of 350bp bandpattern. Cell lines designed as C. catla bloodmonocyte was developed from adherent peripheralfish are one of the healthiest and low calorie nutrientrich food sources. Partial sequencing, amplificationand alignment of fragments of two mitochondrialgenes 16S rRNA and CO1 confirmed that thedeveloped cell line originated from C. catla. Thiscell line could be successfully employed forcytotoxicity assays as revealed by uptake of neutralred. This cell line can be auseful tool to study the roleof macrophages in teleost immune response anddevelopment of markers for macrophages.
KEYWORDS:Catlacatla;Edwardsila;Aeromonas; Vibriosps; RFLP;ELISA