Analysis of the SOD1 Gene in Keratoconus Patients from Saudi Arabia.

Journal Article

KK., Al-Muammar AM, Kalantan H, Azad TA, Sultan T, Abu-Amero . 2014

• Publication Work Type: Research
• Magazine \ Newspaper: Ophthalmic Genet.
• Issue Number: 4
• Volume Number: 36
• Pages: 373-5

Publication Abstract

We investigated Saudi patients with familial and sporadic Keratoconus for mutations in the Superoxide dismutase 1, soluble (SOD1) gene. We sequenced the entire coding region, exon-intron boundaries and intron 2 encompassing a 7-bp deletion in clinically confirmed Keratoconus patients (n = 55) and 100 ethnically matched healthy controls. All cases and controls were unrelated. Sequencing the SOD1 gene revealed the presence of four nucleotide changes and all were non-coding. Those were g.12035 C4A; g.13978 T4A; g.12037 G4A and g.11931 A4C with similar frequencies in patients and controls. All four sequence changes were benign polymorphisms with no apparent clinical significance. Additionally, the 7-bp deletion in intro2 reported previously, were not detected in any of our Keratocnus cohort. In our Keratoconus cohort, no pathogenic SOD1 mutation(s) was identified.
Keywords: Keratoconus, mutation, Saudi Arabia, SOD1 gene.