Simultaneous determination of monosaccharides and oligosaccharides in dates using liquid chromatography–electrospray ionization mass spectrometry

Journal Article

Al-Shaalan, Ayman A. Ghfar, Saikh M. Wabaidur, A. Yacine Badjah Hadj Ahmed, Zeid A. Alothman, Mohammad R. Khan, Nora H. . 2015

• رابط المنشور على الويب: http://www.sciencedirect.com/science/article/pii/S0308814614019463
• الوسوم: Monosaccharides, Oligosaccharides, Liquid chromatography, Mass spectrometry, Palm dates
• المجلة \ الصحيفة: Food Chemistry
• رقم العدد: 176
• الصفحات: 487–492

مستخلص المنشور

Ultra performance liquid chromatography coupled to mass spectrometry was used for the simultaneous
separation and determination of reducing monosaccharides (fructose and glucose), a non-reducing disaccharide
(sucrose) and oligosaccharides (kestose and nystose) in HILIC mode. The chromatographic separation
of all saccharides was performed on a BEH amide column using an acetonitrile–water gradient
elution. The detection was carried out using selected ion recording (SIR) acquisition mode. The validation
of the proposed method showed that the limit of detection and limit of quantification values for the five
analyzed compounds were in the range of 0.25–0.69 lg/mL and 0.82–3.58 lg/mL, respectively; while the
response was linear in the range of 1–50 lg/mL. The developed method showed potential usefulness for a
rapid and sensitive analysis of underivatized saccharides and was used for determination of sugars in
three date samples (Sefri, Mabroom, Ghassab) which were soxhlet extracted by ethanol