Nucleotide sequence polymorphism within exon 3 region of leptin and prolactin genes in Herri sheep
Mahmoud, Ahmed Hossam . 2014
The objective of the present study was to determine the polymorphism in the leptin (LEP) and prolactin (PRL) genes in Herri (n=35) sheep breed of Saudi Arabia. No single nucleotide polymorphism (SNP) was detected in the 156-bp PCR amplicon of PRL exon 3 region. The aligned DNA sequences of the 471-bp fragment of LEP exon 3 showed four non-synonymous SNPs and two synonymous SNPs. The non-synonymous SNPs were found at positions 170 (GGG/AGG-transition), 248 (CTG/CCG-transition), 286 (GTG/TTG-transversion) and 332 (CGG/CAG-transition). The two synonymous SNPs were detected at positions 213 (ACC/GCC transition) and 216 (CCA/CCG transition). The SNP (C) at position 248 was specific to Herri sheep. These sequences were deposited into the GenBank with accession nos. KJ924981-KJ924986. Five genotypes were recognized as homozygotes (01-05) and two were heterozygotes (06 and 07). The most frequent LEP genotype 05 (37%) was also found to be the most common genotype in GenBank database. The present study provided basic information to understand the genetic diversity of Herri sheep breed of Saudi Arabi
ABSTRACT
ABSTRACT
We detected genetic variations and single nucleotide polymorphisms (SNPs) of tyrosinase gene (Tyr) among seven camel
populations in Saudi Arabia. Exon 1 of TYR was amplified…