CLS 331

Department                  :    Clinical Laboratory Sciences

 Course number            :     CLS 331

 Course title                  :     Clinical Biochemistry II   

 

 

 Course description :

             Course work involves a study of the metabolic activity of animal tissue and its regulation. Major catabolic pathways related to carbohydrate, amion acid , and fatty acids are discussed in detail . the study includes a look at the bioenergetics of cells as well as the digestion and absorption of food material .

 

 

CLS   331                              Laboratory Schedule     

 

Week                                        subject

 

1 .                   The hydrolytic activity of salivary amylases on starch  . 

 2 .                   Quantitative determination of amylases activity  .

 3 .                       The production of pyruvate and acetaldehyde 

 4 .                      Identification of free amino acids by TLC using silica gel plates.

 5 .                     Quantitative determination of protein by the biuret .

 6 .                   Enzymatic Digesiton of fat by pancreatic lipase . 

 7 .                  Effect of Bile salts on pancreatic lipase activity .

 8 .                   Determination of serum urea by the berthelot reaction .

 9 .                   The determination of the phosphorus content of a nucleic acid .

 10 .                 Determination of inorganic phosphate . 

 11 .                Determination of the glycogen content in liver .

 12 .                 Revision

 13 .                 FINAL LABORATORY EXAMINATION  . 

 

 

lab Work assessment :

Semester :15

Lab Final Exam:15

 

References:

Clinical Biochemistry: An Illustrated Colour Text
By Allan Gaw, Robert A., Ph.D. Cowan, Denis St. J., M.D. O'Reilly, Michael J., Ph.D. Stewart, James Shepherd