S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells

مقال فى مجلة

2022

تم النشر فى: https://doi.org/10.1016/j.jksus.2021.101735

Plants have been used in the treatment of many diseases. The aim of the present investigation was to
assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant
was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant
activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined
using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using
DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and
the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties
with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 lg/
mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic
fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang)
cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and
60 mg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing
of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed
that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and
the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was
highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong
potential as chemopreventive agent in liver cancer.

• رقم المجلد: 34
• مجلة/صحيفة: Journal of King Saud University – Science
• الصفحات: 101735