Larvicidal activity and Histopathological changes of Cinnamomum burmannii, Syzygium aromaticum extracts and their combination on Culex pipiens

مقال فى مجلة

2022

تم النشر فى: https://doi.org/10.1016/j.sjbs.2021.12.035

In order to develop an eco-friendly botanical larvicide alternative to the synthetic larvicides, extracts
were prepared from the Cinnamomum burmannii (C.B.) and Syzygium aromaticum (S.A.) with hexane
using a sonicator. The extracts were evaluated for larvicidal activity individually and in combination
against the Culex pipiens larvae. The LC50 value of C.B. and the S.A. hexane extracts tested individually
were 184.2 and 363.7 mg/mL against Cx. pipiens respectively. All the combinations of the extract of C.B.
and S.A. showed synergistic factors higher than one. Among the different ratios of extracts, the SA25%:
CB75% extract was found to be more toxic than the other combinations (LC50:125.7 mg/mL). Midgut cells
treated with S.A. 25%: C.B. 75% extract showed severe morphological alterations such as degradation of
microvilli; degeneration of epithelial cells, and peritrophic membrane; loss of nuclei, irregular and damage
of microvilli. The extract has a promising larvicidal potential against Cx. pipiens, However, the extract
was toxic against HUVEC cells, as evident from MTT and cell morphology. Further investigation is
required to assess the toxicity of the extract on aquatic animals.

• رقم المجلد: 29
• مجلة/صحيفة: Saudi Journal of Biological Sciences
• الصفحات: 2591–2596