Larvicidal activity and chemical compositions of Aloe ferox mill, and Commipora abyssinica (O.Berg) combination against the mosquito vectors Culex pipiens L

مقال فى مجلة

2022

تم النشر فى: https://doi.org/10.1016/j.jksus.2022.101962

The aqueous extract was prepared from the Aloe ferox, and Commipora abyssinica combined to develop a
botanical mosquito larvicide. The aqueous extract and the solvent fractions obtained using liquid–liquid
extraction were tested against Culex pipiens larvae for larvicidal potential. The maximum larvicidal activity
was recorded for the ethyl acetate (EtOAc) extract with LC50 values of 28.24 mg/mL followed by hexane
(104.42 mg/mL), water (140.24 mg/mL), and chloroform (211.41 mg/mL) extract against the Cx. pipiens third
instar 24 h post-treatment. In midguts of EtOAc extract-treated larvae, Longitudinal sections showed
edema between the degenerated epithelial cells and degraded microvilli. The extract caused a dosedependent
decrease in the percentage of cell viability of HUVEC cells suing MTT (3-[4,5-dimethylthia
zol-2-yl]-2,5-diphenyltetrazolium bromide) assay. The IC50 value of the EtOAc extract was 143.6 mg/mL
and displayed chromosomal condensation. The total phenolic and flavonoid contents calculated were
15.9 GAE/g (gallic acid equivalent per gram) and 3.69 QurE/g (Qurcetin equivalent per gram), respectively.
GC–MS analysis showed that the major chemical components of the EtOAc extract were methyl
ester of hexadecanoic acid (28%), 3-benzylbutanoate (9.4%), methyl ester of octadecanoic acid (8.6%),
and alpha-muurolene (5.3%). The current investigations revealed the possible use of this botanical combination
as a larvicide against Cx. pipiens larvae.

• رقم المجلد: 34
• مجلة/صحيفة: Journal of King Saud University – Science
• الصفحات: 101962