ROS-DEPENDENT APOPTOTIC EFFECT OF Washingtonia filifera H. Wendl. EXTRACTS ON HUMAN BREAST CANCER CELL MCF-7
Washingtonia filifera has many health benefits, but its activity against
cancer cell lines has not yet been explored. The present study assessed the
cytotoxicity of different solvent extracts of W. filifera using MTT
assay. Fluorescence microscopy was used to evaluate apoptosis and
oxidative stress. Only two extracts showed dose- and time-dependent
decrease in cell survival. The ethyl acetate extract of W. filifera (WFET)
exhibited maximum cytotoxicity against HUVECs, MCF7, HCT116, and
HepG2 cells with IC50 values of 21.7, 48.8, 85.8, and 100.6 μg mL-1,
respectively, 24 h post-treatment. Similarly, methanolic extract of W. filifera
(WFME) showed maximum cytotoxicity against HUVECs (IC50: 21.8 μg
mL-1) cells, followed by MCF-7 (73.3 μg mL-1), HCT116 (98.5 μg mL-1), and
HepG2 (100 μg mL-1). Light and florescent microscopy using 4′,6-
diamidino-2-phenylindole, and acridine orange-ethidium bromide dyes
showed apoptotic cell death. Intracellular reactive oxygen species (ROS)
was evaluated using DCFH-DA dye. The WFET and WFME extracts showed
increased intracellular ROS with increased concentrations of the two
extracts using MCF7 cells. Overall, the WFME and WFET extracts could
act as promising chemo-preventive agents against breast carcinoma cells.
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