SYNTHETIC SEED PRODUCTION AND PHYSIO-BIOCHEMICAL STUDIES IN CASSIA ANGUSTIFOLIA VAHL. – A MEDICINAL PLANT
, Najat A. Bokhari . 2014
Synthetic seed technology is an alternative to traditional micropropagation for production and delivery of
cloned plantlets. Synthetic seeds were produced by encapsulating nodal segments of
C. angustifolia
in
calcium alginate gel. 3% (w/v) sodium alginate and 100 mM CaCl
2
∙
2H
2
O were found most suitable for
encapsulation of nodal segments. Synthetic seeds cultured on half strength Murashige and Skoog medi
-
um supplemented with thidiazuron (5.0 μM) + indole-3-acetic acid (1.0 μM) produced maximum number
of shoots (10.9
±
0.78) after 8 weeks of culture exhibiting (78%)
in vitro
conversion response.
Encapsulated nodal segments demonstrated successful regeneration after different period (1–6 weeks) of
cold storage at 4 °C. The synthetic seeds stored at 4 °C for a period of 4 weeks resulted in maximum
conversion frequency (93%) after 8 weeks when placed back to regeneration medium. The isolated shoots
when cultured on half strength Murashige and Skoog medium supplemented with 1.0 μM indole-3-butyr
-
ic acid (IBA), produced healthy roots and plantlets with well-developed shoot and roots were success
-
fully hardened off in plastic pots containing sterile soilrite inside the growth chamber and gradually
transferred to greenhouse where they grew well with 85% survival rate. Growth performance of 2 months
old
in vitro
-raised plant was compared with
in vivo
seedlings of the same age. Changes in the content of
photosynthetic pigments, net photosynthetic rate (P
N
), superoxide dismutase and catalase activity in
C. angustifolia
indicated the adaptation of micropropagated plants to
ex vitro
conditions
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