Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia
The diversity and importance of Echinococcus species in domesticated animals in Saudi
Arabia are poorly understood. In this study, 108 singular (hydatid) cysts were collected from
goats (n = 25), sheep (n = 56) and camels (n = 27). DNA was extracted from the protoscoleces
of individual fertile cysts and used for polymerase chain reaction (PCR) amplification
of mitochondrial subunit 1 of the cytochrome c oxidase 1 (cox1) gene. Amplicon sequencing
results revealed the presence of Echinococcus granulosus sensustricto (s.s.) (genotypes
G1±G3) in 16 of the17 sheep cysts and 2 of the 27 camel cysts.of these samples, 18 (2
camel and 16 sheep) were divided into genotypes G1, G2, and G3.
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